As provided, this is the active form of mouse Granzyme B and not the proform. Activity level is determined by the ability to cleave a synthetic chromogenic Granzyme B substrate. For resuspension and solubility: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8 °C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (e.g., 0.1% BSA) and store in working aliquots at -20 °C to -80 °C.
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SRP3202
Granzyme B from mouse
recombinant, expressed in Hi-5 Insect cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
10 μg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1550,00 zł |
Informacje o tej pozycji
NACRES:
NA.32
UNSPSC Code:
12352200
1550,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
mouse
recombinant
expressed in Hi-5 Insect cells
assay
≥97% (HPLC), ≥97% (SDS-PAGE)
form
lyophilized
mol wt
28.9 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... GZMB(14939)
General description
Granzyme B is a cysteine protease found in the cytoplasmic granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme B is required for the induction of target cell lysis, which occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms. Proteolytic cleavage of substrates by Granzyme B takes place primarily after aspartic acid residues. Recombinant murine Granzyme B is a glycosylated 227 amino acid protein, comprising the mature active portion of the murine Granzyme B precursor. The apparent molecular weight is 28.9 kDa by mass spectrometry.
Biochem/physiol Actions
Granzyme B is a cysteine protease found in the cytoplasmic granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme B is required for the induction of target cell lysis, which occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms. Proteolytic cleavage of substrates by Granzyme B takes place primarily after aspartic acid residues. Recombinant murine Granzyme B is a glycosylated 227 amino acid protein, comprising the mature active portion of the murine Granzyme B precursor. The apparent molecular weight is 28.9 kDa by mass spectrometry.
Physical form
Lyophilized from 1x PBS.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
IIGGHEVKPH SRPYMALLSI KDQQPEAICG GFLIREDFVL TAAHCEGSII NVTLGAHNIK EQEKTQQVIP MVKCIPHPDY NPKTFSNDIM LLKLKSKAKR TRAVRPLNLP RRNVNVKPGD VCYVAGWGRM APMGKYSNTL QEVELTVQKD RECESYFKNR YNKTNQICAG DPKTKRASFR GDSGGPLVCK KVAAGIVSYG YKDGSPPRAF TKVSSFLSWI KKTMKSS
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Ta pozycja | |||
|---|---|---|---|
| description recombinant, expressed in Hi-5 Insect cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture | description recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture | description recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture | description recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture |
| recombinant expressed in Hi-5 Insect cells | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| assay ≥97% (HPLC), ≥97% (SDS-PAGE) | assay ≥98% (HPLC), ≥98% (SDS-PAGE) | assay ≥98% (HPLC), ≥98% (SDS-PAGE) | assay ≥98% (HPLC), ≥98% (SDS-PAGE) |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| mol wt 28.9 kDa | mol wt 16.8 kDa | mol wt 26.2 kDa | mol wt 14.3 kDa |
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Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Hello, Is this enzyme in its active form? What are the best condition for resuspension? Thank you.
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