Activity units (U or U/µg) are not available for this product. MMP-2 activity was measured by its ability to cleave a chromogenic peptide substrate at room temperature. At an MMP-2 concentration of 2.5 µg/mL, 50% cleavage was achieved at an incubation time of approximately 25 minutes.
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SRP3118
MMP-2 human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonim(y):
Gelatinase A
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
10 μg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1870,00 zł |
Informacje o tej pozycji
NACRES:
NA.32
UNSPSC Code:
12352204
Assay:
≥98% (HPLC), ≥98% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli
1870,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC), ≥98% (SDS-PAGE)
form
lyophilized
mol wt
62.0 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... MMP2(4313)
General description
Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. MMP-2 is a secreted collagenase with specificity toward Type IV, V, VII, and X collagens. The gene is mapped to human chromosome 16q12.2. Recombinant human MMP-2 is a 62kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain (552 amino acids).
Biochem/physiol Actions
Matrix metalloproteinases (MMPs) enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, which has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. High levels of MMP-2 are observed in the acute phase of transverse myelitis. It is upregulated in colorectal carcinoma and is associated with poor survival outcome. MMP-2 gene haplotypes are also linked with risk of thoracic aortic dissection, a cardiovascular disorder with high death rate.
Physical form
Lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 0.1 mM Calcium Chloride.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
MYNFFPRKPK WDKNQITYRI IGYTPDLDPE TVDDAFARAF QVWSDVTPLR FSRIHDGEAD IMINFGRWEH GDGYPFDGKD GLLAHAFAPG TGVGGDSHFD DDELWTLGEG QVVRVKYGNA DGEYCKFPFL FNGKEYNSCT DTGRSDGFLW CSTTYNFEKD GKYGFCPHEA LFTMGGNAEG QPCKFPFRFQ GTSYDSCTTE GRTDGYRWCG TTEDYDRDKK YGFCPETAMS TVGGNSEGAP CVFPFTFLGN KYESCTSAGR SDGKMWCATT ANYDDDRKWG FCPDQGYSLF LVAAHEFGHA MGLEHSQDPG ALMAPIYTYT KNFRLSQDDI KGIQELYGAS PDIDLGTGPT PTLGPVTPEI CKQDIVFDGI AQIRGEIFFF KDRFIWRTVT PRDKPMGPLL VATFWPELPE KIDAVYEAPQ EEKAVFFAGN EYWIYSASTL ERGYPKPLTS LGLPPDVQRV DAAFNWSKNK KTYIFAGDKF WRYNEVKKKM DPGFPKLIAD AWNAIPDNLD AVVDLQGGGH SYFFKGAYYL KLENQSLKSV KFGSIKSDWL GC
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Ta pozycja | |||
|---|---|---|---|
| description recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture | description affinity isolated antibody | description >90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder | description clone A-Gel VC2, Chemicon®, from mouse |
| biological source human | biological source rabbit | biological source - | biological source mouse |
| Gene Information human ... MMP2(4313) | Gene Information human ... MMP2(4313) | Gene Information human ... MMP2(4313) | Gene Information human ... MMP2(4313) |
| assay ≥98% (HPLC), ≥98% (SDS-PAGE) | assay - | assay >90% (SDS-PAGE) | assay - |
| technique(s) cell culture | mammalian: suitable | technique(s) western blot: 1:500-1:1000, ELISA: 1:20000 | technique(s) - | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, ELISA: suitable |
| form lyophilized | form buffered aqueous solution | form lyophilized powder | form - |
| recombinant expressed in E. coli | recombinant - | recombinant expressed in NSO cells | recombinant - |
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Klasa składowania
11 - Combustible Solids
Co się dzieje?
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Wykazy regulacyjne
Wykazy regulacyjne dotyczą głównie produktów chemicznych. Można w nich podawać ograniczoną liczbę informacji na temat produktów niechemicznych. Brak wpisu oznacza, że żaden ze składników nie znajduje się w wykazie. Użytkownik odpowiada za zagwarantowanie bezpiecznego i zgodnego z prawem stosowania produktu.
10043-52-4
CAS No.
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Dear Technical Support, Could you provide the activity units (U or U/µg) for MMP-2 (SRP3118)? If the unit value is not available, please advise how the enzyme activity should be evaluated, including any recommended substrates or assay methods.
1 answer-
Helpful?
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Can you confirm if this product is an activated form of MMP-2, or does it require activation with APMA?
1 answer-
The product SRP3118 does not need activation with APMA since it is not a pro-MMP; it is expressed as the mature sequence.
Helpful?
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What is the test method for Product No. SRP3118-10UG, MMP-2 Recombinant Human?
1 answer-
The test method for Product No. SRP3118-10UG, MMP-2 Recombinant Human involves measuring the MMP-2 human bioactivity by its ability to cleave a chromogenic peptide MMP-2 substrate at room temperature. This was done at an MMP-2 concentration of 2.5 ug/ml, achieving 50% cleavage at an incubation time of approximately 25 minutes. The protocol specifies using a kit supplied by AnaSpec (Catolog#72095), which includes diluting the colorimetric substrate, reconstituting the MMP-2 sample with water, mixing the MMP-2 and substrate solution, and taking readings at 412nm every 10 minutes for about 90 minutes or until a steady reading is obtained.
Helpful?
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