SRP0712

EGFR (C797S), Active

recombinant

• Kod UNSPSC: 12352202
• NACRES: NA.77

Właściwości

• rekombinowane: expressed in baculovirus infected Sf9 cells
• Próba: 70-95% (SDS-PAGE)
• Postać: frozen liquid
• aktywność właściwa: 74-102 nmol/min·mg (radiometric)
• masa cząsteczkowa: ~89-100 kDa
• stężenie: 0.1 μg/μL
• nr dostępu: NM_005228
• Warunki transportu: dry ice
• temp. przechowywania: −70°C
• informacje o genach: human ... EGFR(1956)

Opis

Zastosowanie

Useful for kinase assay.

Działania biochem./fizjol.

Recombinant human EGFR (C797S) (668-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility, and cell survival ⁽¹⁾. Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms ⁽²⁾. Activation of EGFR triggers activation of the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation.

Rekonstytucja

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Przechowywanie i stabilność

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable