Ludzki DYRK2 (GenBank Accession No. NM_003583), pełnej długości z N-końcowym znacznikiem His, MW = 63,5 kDa, wyrażony w systemie ekspresji komórek Sf9 zakażonych bakulowirusem.
Zastosowanie
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Postać fizyczna
TBST+20% glicerol+3mM DTT
Uwaga dotycząca przygotowania
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
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Cellular and molecular life sciences : CMLS, 77(13), 2621-2639 (2019-10-13)
NOTCH proteins constitute a receptor family with a widely conserved role in cell cycle, growing and development regulation. NOTCH1, the best characterised member of this family, regulates the expression of key genes in cell growth and angiogenesis, playing an essential
Autophosphorylation of amino-acid residues is part of the folding process of various protein kinases. Conventional chemical screening of mature kinases has missed inhibitors that selectively interfere with the folding process. Here we report a cell-based assay that evaluates inhibition of
Rapidly increasing availability of genomic data and ensuing identification of disease associated mutations allows for an unbiased insight into genetic drivers of disease development. However, determination of molecular mechanisms by which individual genomic changes affect biochemical processes remains a major
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