I have extensively used these lentiviral vectors to perform KD experiments, and they work. We have performed experiments in different cell types, from macrophages to epithelial cells, from both mice and humans. I totally recommend it.
Przejdź do
SHCLNV
MISSION® shRNA
Lentiviral Particles
MISSION® shRNA
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, aby wyświetlić ceny organizacyjne i kontraktowe.
Informacje o tej pozycji
UNSPSC Code:
41106609
Technique(s):
capture ELISA: 106 VP/mL using p24
To order shRNA please search for your gene of interest.
General description
Our comprehensive shRNA product offering consists of over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes. The library is available in: gene family sets: gene collections related to specific functional classes such as kinases and ion channels; gene taget sets, sets of shRNA clones that target your favorite gene; and individual shRNA clones. Our shRNA and lentiviral manufacturing platforms utilize dedicated laboratories featuring state-of-the-art robotics, liquid handling and laboratory information management systems (LIMS) to provide superior quality and service for your RNAi research needs.
Lentiviral particles are ideal for transducing a wide range of cell lines. VSV-G Psuedotyping of the MISSION® lentiviral particles allows for entry into a wide range of cells. In addition, lentiviral particles stably integrate the shRNA into the genomes of both dividing and non-dividing cell lines. The MISSION bioproduction team regularly produces high quality lentivirus, so that our customers can get straight to their RNAi experiment without wasting precious time on tedious lentiviral production. Our minimum titer is 106 VP/mL. Unlike adenoviral infections that require vast excesses, lentiviral transduction is efficient enought allow for MOI as low as 1.
Application
MISSION® shRNA has been used:
- in transfections and transductions
- in the establishment of stable cell lines expressing shRNA
- in the generation of alpha-actinin-4 (ACTN4) knockdown cell line
- to transduce raw 264.7 cells[1]
Other Notes
To see protocols and application data please visit sigma.com/shrna.
Legal Information
Use of this product is subject to one or more license agreements.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
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Ta pozycja | |||
|---|---|---|---|
| technique(s) capture ELISA: 106 VP/mL using p24 | technique(s) capture ELISA: 106 VP/mL using p24 | technique(s) capture ELISA: 5 x 108 TU/mL using p24 | technique(s) capture ELISA: 5 x 108 TU/mL using p24 |
| storage temp. −70°C | storage temp. −70°C | storage temp. −70°C | storage temp. −70°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Klasa składowania
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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