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S4521

Saponin from Quillaja sp.

Sapogenin content 20-35 %

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Rozmiar/SKUDostępnośćCena netto
10 g
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
590,00 zł
25 g
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
1190,00 zł

Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
12161902
NACRES:
NA.32
EC Number:
232-462-6
MDL number:

590,00 zł


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description

non-ionic

Quality Segment

composition

Sapogenin content, 20-35%

technique(s)

HPLC: suitable, protein quantification: suitable

sulfated ash

≤10%

solubility

soluble (Water solubility: 2.000 g/l at 19,5 °C - OECD Test Guideline 105 - completely)

SMILES string

O1[C@H]([C@@H]([C@H]([C@@H]([C@H]1CO)O)O)O)O[C@H]2[C@@H](O[C@@H]([C@H]([C@@H]2O)O[C@@H]8O[C@@H]([C@H]([C@@H]([C@H]8O)O)O)CO)C(=O)O)O[C@@H]3[C@@](C4[C@@](C5[C@]([C@@]6(C[C@H]([C@]7([C@H]([C@@H](C(CC7C6=CC5)(C)C)OC(=O)\C(=C/C)\C)OC(=O)C)CO)O)C)(CC4)C)(CC3)C

InChI

1S/C55H86O24/c1-10-23(2)46(71)79-43-44(72-24(3)60)55(22-59)26(17-50(43,4)5)25-11-12-30-51(6)15-14-32(52(7,21-58)29(51)13-16-53(30,8)54(25,9)18-31(55)61)75-49-41(77-48-38(67)36(65)34(63)28(20-57)74-48)39(68)40(42(78-49)45(69)70)76-47-37(66)35(64)33(62)27(1

InChI key

AXNVHPCVMSNXNP-ZELRDNAQSA-N

General description

Saponins are steroid or triterpenoid glycosides. They are biosurfactants that have several uses in the food and cosmetic industry.

Application

Saponin from Quillaja bark has been used:
  • to stain fertilized oocytes
  • in the analysis of intracellular cytokine production
  • to permeabilize dendritic cells

Biochem/physiol Actions

Saponins solubilize the low density lipoprotein (LDL) fraction of cholesterol in micelles, thus lowering their concentration. Saponin from Quillaja bark is used as a flavoring agent in food and beverages. This saponin has been shown to enhance immune-cell proliferation in vitro.
Potent hemolytic when injected i.v.; surfactant that enhances penetration of proteins and other macromolecules through cell membranes; it also has been used as an adjuvant for vaccines.

Preparation Note

Purified to remove low molecular weight contaminants 
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Ta pozycja
558255SAE0073D1685
Saponin A glycoside used to permeabilize cellular membranes. Exhibits hemolytic activity.

Millipore

558255

Saponin

description

Sapogenin content 20-35 %

description

A glycoside used to permeabilize cellular membranes. Exhibits hemolytic activity.

description

-

description

meets USP testing specifications

technique(s)

HPLC: suitable, protein quantification: suitable

technique(s)

-

technique(s)

LC/MS: suitable, protein quantification: suitable, HPLC: suitable

technique(s)

protein quantification: suitable

Quality Level

300

Quality Level

100

Quality Level

200

Quality Level

200

solubility

soluble (Water solubility: 2.000 g/l at 19,5 °C - OECD Test Guideline 105 - completely)

solubility

water: soluble

solubility

-

solubility

-

description

non-ionic

description

Merck USA index - 14, 8365

description

non-ionic

description

anionic

sulfated ash

≤10%

sulfated ash

-

sulfated ash

≤10%

sulfated ash

-

composition

Sapogenin content, 20-35%

composition

-

composition

Sapogenin content, 20-35%

composition

-


pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Klasa składowania

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves



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Lot/Batch Number

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Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. Is this product suitable for downstream molecular tests if used in a lysis buffer for DNA extraction ?

    1 answer
    1. This product has not been tested for suitability with any molecular tests after being used in a DNA lysis buffer. Validation by the intended user is necessary, as this has not been assessed. Researchers associated with Radboud University Medical Center have reported the usage of product 47036, or a molecular biology grade saponin, for bacterial DNA isolation prior to qPCR. For more details, refer to the citation below.

      Optimized DNA isolation method for microbiome analysis of human tissues
      Carlijn Bruggeling, Daniel R. Garza, Soumia Achouiti, Wouter Mes, Bas E. Dutilh, Annemarie Boleij
      bioRxiv 2020.08.25.267641; doi: https://doi.org/10.1101/2020.08.25.267641

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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