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S1452
MISSION® siRNA Transfection Reagent
Synonim(y):
MISSION® siRNA Transfection Reagent
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Informacje o tej pozycji
NACRES:
NA.51
UNSPSC Code:
41106502
General description
The MISSION® siRNA Transfection Reagent is for the transfection of siRNA to achieve ≥90% silencing efficiency for transient knockdown of eukaryotic gene expression.
Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.
Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.
Other Notes
Gene silencing assay: An A549 cell line stably expressing firefly luciferase was transfected in 24-well plates with 8.4 ng of 21-mer Luciferase siRNA using 2 μL of the MISSION® siRNA Transfection Reagent. Experiments were performed in triplicate in presence of serum. Cell lysates were assessed for luciferase expression 48 hours post-transfection.
Legal Information
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
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Ta pozycja | |||
|---|---|---|---|
| product line MISSION® | product line - | product line MISSION® | product line MISSION® |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −70°C | storage temp. −70°C |
| form aqueous solution | form liquid | form - | form - |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
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Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Klasa składowania
3 - Flammable liquids
wgk
WGK 3
flash_point_f
120.2 °F
flash_point_c
49 °C
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