RNB500
GenElute™ Plasma/Serum RNA Purification Mini Kit
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About This Item
Kod UNSPSC:
41105600
charakterystyka ekologicznej alternatywy
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sustainability
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kategoria ekologicznej alternatywy
, Aligned
Warunki transportu
ambient
temp. przechowywania
room temp
Opis ogólny
Sigma′s GenElute™ Plasma/Serum RNA Purification Mini Kit provides a fast, reliable, reproducible and simple procedure for isolating circulating RNA and exosomal RNA from small plasma/serum inputs ranging from 10 μL to 25 μL, with various kit formats addressing different plasma/serum input volumes. Purification is based on spin column chromatography that uses Sigma′s proprietary resin separation matrix. The kit is designed to isolate all sizes of circulating RNA, including microRNA, as well as all sizes of exosomal RNA.
Sigma′s GenElute™ Plasma/Serum RNA Purification Mini Kit provides a clear advantage over other available kits in that they do not require phenol/chloroform or any protease treatments. RNA can be isolated from either fresh or frozen samples using these kits. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 10 μL to 100 μL. Typical yields of free-circulating and exosomal RNA vary depending on the input sample, as the amount of RNA present in plasma and serum will depend upon the health status of the individual.
Sigma′s GenElute™ Plasma/Serum RNA Purification Mini Kit provides a clear advantage over other available kits in that they do not require phenol/chloroform or any protease treatments. RNA can be isolated from either fresh or frozen samples using these kits. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 10 μL to 100 μL. Typical yields of free-circulating and exosomal RNA vary depending on the input sample, as the amount of RNA present in plasma and serum will depend upon the health status of the individual.
- Isolate all sizes of circulating and exosomal RNA, including microRNA
- Versatile plasma/serum input ranging from 50 μL to 200 μL
- No phenol extractions
- No carrier RNA
- Concentrate circulating RNA and Exosomal RNA into a flexible elution volume ranging from 10 μL to 25 μL
- Purify high-quality RNA in 15-20 minutes
Zastosowanie
- Reverse transcription qPCR
- Reverse transcription PCR
- Northern blotting
- RNase protection and primer extension
- Expression array assays.
- miRNA array
- RNA-Seq
- Nanostring
- Fluidigm
- Droplet/Digital PCR
Działania biochem./fizjol.
GenElute™ Plasma/Serum RNA Purification Mini Kit has been used for extraction of total serum RNA from human hepatocellular carcinoma (HCC) and colorectal cancer (CRC) samples.
Definicja jednostki
Preps
Informacje prawne
GenElute is a trademark of Sigma-Aldrich Co. LLC
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Kod klasy składowania
12 - Non Combustible Liquids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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