RES2042D

3,3′-Diaminobenzidine tetrahydrochloride hydrate

≥98.0% (titration with NaOH)

• Synonim(y): DAB tetrahydrochloride hydrate, [1,1′-Biphenyl]-3,3′,4,4′-tetraamine tetrahydrochloride hydrate
• Wzór liniowy: C₁₂H₁₄N₄ · 4HCl · xH₂O
• Numer CAS: 868272-85-9
• UNSPSC Code: 12171500
• NACRES: NA.21
• EC Number: 231-018-9
• MDL number: MFCD08273058

Właściwości

• Quality Segment: 300
• assay: ≥98.0% (titration with NaOH)
• form: powder
• mol wt: 360.11 g/mol
• concentration: ≤100% (Biphenyl-3,3′,4,4′-tetrayltetraammonium tetrachloride)
• color: white to faint red
• storage temp.: −20°C
• SMILES string: O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
• InChI: 1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2
• InChI key: DXWSCXIZIHILNP-UHFFFAOYSA-N

Opis

General description

3,3′-Diaminobenzidine tetrahydrochloride hydrate is a chromogen commonly used in immunohistochemistry and other staining techniques to visualize the presence of antigens or antibodies in biological samples. DAB HCl is designed as an immunohistology stain to visualize peroxidase activity in cells. DAB oxidation can be measured using a spectrophotometer.

Application

3,3′-Diaminobenzidine tetrahydrochloride hydrate is suitable for immunohistology staining procedures, western blotting, and FISH (fluorescence in situ hybridization). DAB is used in studying epithelial cell apoptosis in the event of early cataracts, and immunohistochemistry protocols of autosomal dominant polycystic kidney disease (PKD).

Biochem/physiol Actions

In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown-colored complex.

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Informacja o środkach bezpieczeństwa

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H302,H319,H341,H350
• pcodes: P202 - P264 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2
• Klasa składowania: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• flash_point_f: Not applicable
• flash_point_c: Not applicable