R8507

Hpa I from Haemophilus parainfluenzae

Restriction Enzyme

• Numer CAS: 81295-24-1
• UNSPSC Code: 12352204
• MDL number: MFCD00166572
• Numer WE: 3.1.21.4 (BRENDA, IUBMB)

Właściwości

• grade: Molecular Biology
• form: buffered aqueous glycerol solution
• concentration: 3,000-10,000 units/mL
• shipped in: wet ice
• storage temp.: −20°C

Opis

Application

HpaI is a restriction endonuclease that is used to cleave DNA at the recognition site 5′-GTT/AAC-3′, generating fragments with blunt ends.

Biochem/physiol Actions

Recognition sequence: 5′-GTT/AAC-3′
Ligation and recutting results: After 2-10-fold Hpa I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >95% recut.
Heat inactivation: Not completely inactivated at 65 °C for 15 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.5 mM EDTA, 5 mM 2-mercaptoethanol, 0.01% polydocanol, 50% glycerol (v/v) at 4°C

Other Notes

Supplied with 10x Restriction Enzyme Buffer SA (B7531).

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• Co się dzieje?: WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable