R1258

Kpn I from Klebsiella pneumoniae

Restriction Enzyme

• Numer CAS: 81295-27-4
• UNSPSC Code: 12352204
• MDL number: MFCD00166575
• Numer WE: 3.1.21.4 (BRENDA, IUBMB)

Właściwości

• grade: Molecular Biology
• form: buffered aqueous glycerol solution
• concentration: 10,000 units/mL
• shipped in: wet ice
• storage temp.: −20°C

Opis

Application

KpnI is a DNA restriction enzyme that is used in molecular biology research to cleave DNA at the recognition sequence 5′-GGTAC/C-3′ to generate DNA fragments with 3′-cohesive termini.

Biochem/physiol Actions

Recognition sequence: 5′-GGTAC/C-3′
Cutting results: a 2-10-fold Kpn I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 50 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% polydocanol (v/v) at 4°C

Other Notes

Comment: Kpn I shows abnormal DNA cleaving patterns (star activity) in reactions containing high (>5%) glycerol, >5% (v/v); excess enzyme and high pH (>8.0).
The enzyme will not cleave DNA containing 3′-residue of 5-methylcytosine.

Supplied with 10x Restriction Enzyme Buffer SL (B3782)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable