R0884

T7 RNA Polymerase

recombinant, expressed in E. coli, buffered aqueous solution

• Synonim(y): RNA Polymerase T7, RNA Polymerase, T7 from E. coli HMS 174/pAR1219
• Numer CAS: 9014-24-8
• Numer EC enzymu: 2.7.7.6 (BRENDA, IUBMB)
• Numer MDL: MFCD00132197
• Kod UNSPSC: 12352204

Właściwości

• rekombinowane: expressed in E. coli
• klasa czystości: for molecular biology
• Postać: buffered aqueous solution
• masa cząsteczkowa: 98.8 kDa
• stężenie: 10,000-50,000 U/mL
• numer dostępu UniProt: P00573
• obecność zanieczyszczeń: DNase and RNase, none detected
• temp. przechowywania: −20°C
• informacje o genach: bacteriophage T7 ... T7p07(1261050)

Opis

Opis ogólny

T7 RNA polymerase is highly specific for the bacteriophage T7 promoter and terminator sequences. It is extensively used to prepare RNA transcripts for stuctural and metabolic studies. The RNA transcripts can be converted to probes for sensitive hybridization detection studies. T7 polymerase and dideoxynucleotides can be used to directly sequence DNA.

Komponenty

T7 RNA Polymerase is supplied as a solution of 100 mM NaCl, 50 mM Tris-HCl (pH 7.9), 0.1 mM EDTA, 0.1% Triton X-100, 1 mM DTT, and 50% (v/v) glycerol.

Definicja jednostki

One unit will catalyze the incorporation of 1 nmol of rNTP into acid-precipitable material in 60 min at 37°C.

Komentarz do analizy

Activity assay: 40 mM Tris-HCl, pH 7.9, 6 mM MgCl₂, 4 mM spermidine, 10 mM DTT, 0.5 μM each rNTP + 10 μCi α-³²P-UTP, 3-10 units of enzyme, and 1 μg of a 350 bp template are incubated for 10 min at 37°C in a total volume of 100 μl. Typical results are ≥50% incorporation of labeled nucleotide into ≥90% full-length transcript.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable