P9249

Monoclonal Anti-p53−Biotin antibody produced in mouse

~1 mg/mL, clone DO-1, ascites fluid, buffered aqueous solution

• Numer MDL: MFCD01864735
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• białko sprzężone: biotin conjugate
• forma przeciwciała: ascites fluid
• rodzaj przeciwciała: primary antibodies
• klon: DO-1, monoclonal
• Postać: buffered aqueous solution
• masa cząsteczkowa: antigen ~53 kDa
• reaktywność gatunkowa: human
• stężenie: ~1 mg/mL
• metody: flow cytometry: suitable; immunocytochemistry: 5-10 μg/mL using human A431; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.5-1 μg/mL using human A431 cell extracts
• izotyp: IgG2a
• numer dostępu UniProt: P04637
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... TP53(7157)

Opis

Opis ogólny

p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.

Immunogen

recombinant human wild type p53. The epitope resides beween amino acids 20-25 of human p53.

Zastosowanie

Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for Western Blotting.

Działania biochem./fizjol.

Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Przechowywanie i stabilność

For continuous use, store at 2-8°C for up to one month.
For extended storage, freeze in working aliquots.
Repeated freezing and thawing, or storage in “frostfree”freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Inne uwagi

In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)