P5400

Pectinesterase from orange peel

Name: Pectinesterase from orange peel
Brand: SIGMA
Price: 4590 PLN

lyophilized powder, ≥150 units/mg protein

Synonim(y):

Pectin methylesterase, Pectin pectylhydrolase

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P5400-1KU

4590,00 zł

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Informacje o tej pozycji

Numer CAS:

9025-98-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-807-0

MDL number:

MFCD00131812

Numer WE:

3.1.1.11 (BRENDA, IUBMB)

Specific activity:

≥150 units/mg protein

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form

lyophilized powder

Quality Level

200

specific activity

≥150 units/mg protein

composition

Protein, 20-50% biuret

greener alternative product characteristics

Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

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greener alternative category

, Enabling

storage temp.

2-8°C

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Ta pozycja	P5936	P2401	C1794
Sigma-Aldrich P5400 Pectinesterase from orange peel	Sigma-Aldrich P5936 Pectolyase from Aspergillus japonicus	Sigma-Aldrich P2401 Pectinase from Rhizopus sp.	Sigma-Aldrich C1794 Cellulase from Trichoderma sp.
specific activity ≥150 units/mg protein	specific activity ≥0.3 unit/mg solid	specific activity 400-800 units/g solid	specific activity 3-10 units/mg solid
form lyophilized powder	form lyophilized powder	form powder	form powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
composition Protein, 20-50% biuret	composition -	composition -	composition -
greener alternative product characteristics Design for Energy Efficiency Learn more about the Principles of Green Chemistry.	greener alternative product characteristics Design for Energy Efficiency Learn more about the Principles of Green Chemistry.	greener alternative product characteristics Waste Prevention Design for Energy Efficiency Learn more about the Principles of Green Chemistry.	greener alternative product characteristics Design for Energy Efficiency Learn more about the Principles of Green Chemistry.

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.

Application

The enzyme from Sigma has been used as a standard during the measurement of pectin methylesterase activity in sea buckthorn juices. It has been used in the hydrolysis of cell wall pectins while studying phyllotaxis in Arabidopsis.[1] It has also been used to catalyze pectin hydrolysis (using an ultrasonic measurement device) for monitoring biological processes.

Pectinesterase is used to hydrolyze methyl esters of pectin to pectate and methanol. It contains pectin methylesterase and pectin pectylhydrolase. Product P5400 is from orange peel and comes as a lyophilized powder. It contains (NH₄)₂SO₄ and sodium chloride. It has been used to study the pectin methylesterase gene[2].

Biochem/physiol Actions

Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol.

Physical form

Contains (NH₄)₂SO₄ and sodium chloride

Analysis Note

Protein determined by biuret.

Other Notes

One unit will release 1.0 microequivalent of acid from pectin per min at pH 7.5 at 30 °C.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

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Klasa składowania

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Arabidopsis phyllotaxis is controlled by the methyl-esterification status of cell-wall pectins.

Alexis Peaucelle et al.

Current biology : CB, 18(24), 1943-1948 (2008-12-23)

Plant organs are produced from meristems in a characteristic pattern. This pattern, referred to as phyllotaxis, is thought to be generated by local gradients of an information molecule, auxin. Some studies propose a key role for the mechanical properties of

On-line characterization using ultrasound of pectin hydrolysis catalyzed by the enzyme pectinmethylesterase.

Cristina A, et al.

IOP Conference Series: Materials Science and Engineering, 42 (2012)

Separation of Arabidopsis pollen tetrads is regulated by QUARTET1, a pectin methylesterase gene.

Kirk E Francis et al.

Plant physiology, 142(3), 1004-1013 (2006-09-19)

Arabidopsis (Arabidopsis thaliana) QUARTET (QRT) genes are required for pollen separation during normal floral development. In qrt mutants, the four products of microsporogenesis remain fused and pollen grains are released as tetrads. In Arabidopsis, tetrad analysis in qrt mutants has

Pectinesterase Activity and Proximate Analyses of Sea Buckthorn Juices

Harrison JE and Beveridge THJ

Journal of Food Science, 71(1), C23-C27 (2006)

Plant cell wall homeostasis is mediated by brassinosteroid feedback signaling.

Sebastian Wolf et al.

Current biology : CB, 22(18), 1732-1737 (2012-08-14)

Brassinosteroid (BR) signaling is required for normal plant growth as shown by the dwarf phenotype of loss-of-function BR biosynthetic or perception mutants. Despite a detailed understanding of the BR signaling network, it is not clear how exactly BRs control growth.

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Complex Carbohydrate Analysis- Enzymes, Kits and Reagents

(P5400) - Enzyme Assay

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