P4408

Poly-D-lysine hydrobromide

mol wt 15,000-30,000

• Wzór liniowy: D-Lys-(D-Lys)_n-D-Lys · xHBr
• Numer CAS: 27964-99-4
• UNSPSC Code: 12352200
• PubChem Substance ID: 24898537
• MDL number: MFCD00131934

Właściwości

• mol wt: 15,000-30,000
• storage temp.: −20°C
• SMILES string: O=C(C)[C@@](NC)([H])CCCCN.[Br]
• InChI: 1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H
• InChI key: MEXAGTSTSPYCEP-UHFFFAOYSA-N

Opis

Application

Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm². Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

Poly-D-lysine (PDL) hydrobromide is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.

Analysis Note

Molecular weight based on viscosity.

Other Notes

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the β structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Disclaimer

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Klasa składowania: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)