P3752

Phosphatase, Acid from potato

lyophilized powder, ≥0.5 unit/mg solid

• Synonim(y): (Orthophosphoric-monoester phosphohydrolase acid optimum), Acid Phosphatase from potato
• Numer CAS: 9001-77-8
• eCl@ss: 42010102
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-630-9
• MDL number: MFCD00131847
• Numer WE: 3.1.3.2 (BRENDA, IUBMB)
• Specific activity: ≥0.5 unit/mg solid
• Biological source: potato

Właściwości

• biological source: potato
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥0.5 unit/mg solid
• mol wt: 69 kDa
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C
• SMILES string: O(CC(O)C)CC#C
• InChI: 1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
• InChI key: GZCWLCBFPRFLKL-UHFFFAOYSA-N

Opis

Application

Acid phosphatase is routinely used to dephosphorylate proteins and nucleic acids. Acid phosphatase is an acid hydrolase that normally resides in lysosomes. It is used as marker for the identification of lysosomes in subcellular fractionations. Acid phosphatase, product P3752, has been used as a control in order to study how Mycobacterium bovis BCG effects the levels of acid phosphatase activity in alveolar macrophages[1].

Biochem/physiol Actions

Acid phosphatase is a cytoplasmic lysosomal hydrolase that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate[1].

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

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Informacja o środkach bezpieczeństwa

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)