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P3644

L-α-Phosphatidylcholine

from soybean, Type IV-S, ≥30% (enzymatic)

Synonim(y):

1,2-Diacyl-sn-glycero-3-phosphocholine, 3-sn-Phosphatidylcholine, L-α-Lecithin, Azolectin, PC

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Do Państwa/SKUDostępnośćCena netto
25 g
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492,00 zł
100 g
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1480,00 zł
500 g
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5850,00 zł
1 kg
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8960,00 zł

Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
51321705
NACRES:
NA.25
EC Number:
232-307-2
Beilstein/REAXYS Number:
5209585

492,00 zł


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biological source

soybean

Quality Segment

type

Type IV-S

form

solid

concentration

≥30% (enzymatic)

solubility

chloroform: soluble 100 mg/mL, clear to slightly hazy, yellow to orange

functional group

phospholipid

lipid type

phosphoglycerides

shipped in

ambient

storage temp.

−20°C

SMILES string

[P](=O)([O-])(OC[C@H](OC(=O)CCCCCCC\C=C/C\C=C/CCCCC)COC(=O)CCCCCCCCCCCCCCC)OCC[N+](C)(C)C

InChI

1S/C42H80NO8P/c1-6-8-10-12-14-16-18-20-21-23-25-27-29-31-33-35-42(45)51-40(39-50-52(46,47)49-37-36-43(3,4)5)38-48-41(44)34-32-30-28-26-24-22-19-17-15-13-11-9-7-2/h14,16,20-21,40H,6-13,15,17-19,22-39H2,1-5H3/b16-14-,21-20-/t40-/m1/s1

InChI key

JLPULHDHAOZNQI-ZTIMHPMXSA-N

Application

L-α-Phosphatidylcholine is suitable for use:
  • as a component of human erythroid massive amplification culture (HEMAdef) medium for clinical expansion of human erythroblasts[1]
  • in the chloride efflux assay to examine the chloride permeability of lipid vesicles conferred by soluble CLIC1[2]
  • in an assay to measure solubilized wax synthase activity[3]
  • as a component of buffer A used for the resuspension of the cholate-solubilized catalytic unit of adenylate cyclase[4]
  • as a cryoprotectant for freezing ruminant sperm[5]
  • as a substrate for the estimation of phospholipase activity
  • to make liposomes to assess their efficiency of recruitment of ARF (ADP ribosylation factor) and AP-1 (adaptor protein)[6]
  • in the preparation of the isolated catalytic unit of soluble adenylate cyclase[7]

Biochem/physiol Actions

A major structural phospholipid in brain, comprising approx. 15% of total lipid; primarily localized to gray matter.
Phosphatidylcholine is the major membrane phospholipid in eukaryotic cells, forming the structural component of these membranes. It also serves as a reservoir for several lipid messengers and a source for several bioactive lipids such as, lysophosphatidylcholine, phosphatidic acid, diacylglycerol, lysophosphatidylcholine, platelet activating factor, and arachidonic acid.[8]

Preparation Note

Purified from P 5638
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Ta pozycja
P5638P7443P5394
functional group

phospholipid

functional group

phospholipid

functional group

phospholipid

functional group

phospholipid

biological source

soybean

biological source

soybean

biological source

soybean

biological source

egg yolk (dried)

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

solubility

chloroform: soluble 100 mg/mL, clear to slightly hazy, yellow to orange

solubility

-

solubility

-

solubility

-

form

solid

form

powder or solid

form

lyophilized powder

form

solid

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C


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Klasa składowania

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–4 of 4 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. It is said that it has also been used in cell culture, but is Chloroform or Ether harmless in cell culture? Also, is it completely insoluble in other organic solvents such as Chloroform, Ether, and DMSO?

    1 answer
    1. The product's solubility is tested in chloroform at a concentration of 100 mg/mL. Purified egg L-α-phosphatidylcholine is soluble (100 mg/mL) at room temperature in chloroform, ethanol, and hexane containing 3% ethanol. These solubility characteristics should generally apply to other purified natural product phosphatidylcholine products, such as those from soybean and brain, that contain unsaturated fatty acids. The product's solubility in dimethyl sulfoxide has not been determined. It is advised to check specific cells or cell lines to determine their sensitivity to solvents such as dimethyl sulfoxide, chloroform, and ether.

      Helpful?

  4. What kind of product is it, a powder?

    1 answer
    1. This product is listed as a solid but can range in appearance from waxy to powdery. The consistency is not a tested product specification and is simply reported as a solid. Please see the link below to review the product datasheet for additional information:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P3644-100G0000285416.pdf

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