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NPT01

Sigma-Aldrich

NeuroPorter™ Transfection Kit

Lipid formulation for nucleic acid transfections in neuronal and glial cells

Synonim(y):

NeuroPorter Transfection, Transfection Kit

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About This Item

Kod UNSPSC:
12352200
NACRES:
NA.25

klasa czystości

for molecular biology

Poziom jakości

200

Postać

dried film

zastosowanie

 kit sufficient for 75-200 transfections

dostępność

available only in USA, Canada and EU

metody

transfection: suitable

temp. przechowywania

2-8°C

Opis ogólny

Neuroporter™ Transfection Reagent is a unique formulation of a proprietary cationic lipid optimized for delivery of DNA into primary neurons, glial cells, and cultured neuronal cell lines with high efficiency and low toxicity. The Neuroporter™ Transfection Kit was designed for difficult-to-transfect primary neurons, addressing past problems such as poor cell viability, low transfection efficiency and neuro-degeneration.

Zastosowanie

Suitable for transient and stable transfection of nucleic acids into primary neurons and cultured neuronal cell lines. Use approximately 15-120 μl Neuroporter™ Transfection Reagent and 6-8 μg DNA (in provided unique DNA Dilution buffer when required) per 6 cm cell culture plate. The following cells have been successfully transfected using the Neuroporter™ Transfection Kit:

  • C6 glioma (human)
  • Cortical neurons (rat primary)
  • Dorsal Root Ganglion (DRG) cells (rat)
  • NT2 neurons(human precursor cells)
  • NT neurons (human differentiated cells)
  • Subventricular Zone (SVZ) cells (mouse)
  • White matter cells (mouse)

Cechy i korzyści


  • Optimized for primary neurons, glial cells, and cultured neural cell lines
  • Very low toxicity with no neuro-degeneration or dendrite withdrawal
  • Efficient DNA delivery primary neurons, glial cells, and cultured neural cell lines
  • Fast and easy to use compared to other methods
  • Compatible with both serum and serum-free transfection protocols

Komponenty

1 vial Neuroporter™ Transfection Reagent, dried lipid film (T2823)
1.5 mL Hydration Buffer H9036
7.5 mL DNA Diluent D1941

Przestroga

Do not freeze.

Zasada

A stable, non-covalent complex is formed when the Neuroporter™ Transfection Reagent is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.

Informacje prawne

NeuroPorter is a trademark of Gene Therapy Systems, Inc.
This page may contain text that has been machine translated.

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Kod klasy składowania

12 - Non Combustible Liquids

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Beata Jablonska et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(42), 14775-14793 (2012-10-19)
Diffuse white matter injury (DWMI) caused by hypoxia is associated with permanent neurodevelopmental disabilities in preterm infants. The cellular and molecular mechanisms producing DWMI are poorly defined. Using a mouse model of neonatal hypoxia, we demonstrate a biphasic effect on
Adan Aguirre et al.
Nature, 467(7313), 323-327 (2010-09-17)
Specialized cellular microenvironments, or 'niches', modulate stem cell properties, including cell number, self-renewal and fate decisions. In the adult brain, niches that maintain a source of neural stem cells (NSCs) and neural progenitor cells (NPCs) are the subventricular zone (SVZ)
Beata Jablonska et al.
The Journal of cell biology, 179(6), 1231-1245 (2007-12-19)
We investigated the function of cyclin-dependent kinase 2 (Cdk2) in neural progenitor cells during postnatal development. Chondroitin sulfate proteoglycan (NG2)-expressing progenitor cells of the subventricular zone (SVZ) show no significant difference in density and proliferation between Cdk2(-/-) and wild-type mice
Nikhil G Thaker et al.
Journal of neuroscience methods, 185(2), 204-212 (2009-09-29)
A major challenge for the treatment of cancers, such as glioblastoma multiforme (GBM), has been resistance to radiation and cancer chemotherapeutics. Short interfering RNA (siRNA) based screening may facilitate the identification of genes and pathways essential for cancer cell survival
Simone Di Giovanni et al.
The Journal of biological chemistry, 280(3), 2084-2091 (2004-11-04)
Following spinal cord injury, there are numerous changes in gene expression that appear to contribute to either neurodegeneration or reparative processes. We utilized high density oligonucleotide microarrays to examine temporal gene profile changes after spinal cord injury in rats with
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Transfekcja wprowadza materiał genetyczny do komórek, wspomagając badania nad ekspresją genów i biologią komórek.

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This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

Powiązane treści

Transfection Reagent Selection Guide

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Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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