Each vial of this product contains 1 mg of enzyme, 20 mg of lactose, and 10 mg of PBS buffer salts. One can dissolve 1 mg in 10 mL of water to create aliquots. The concentration of the aliquots does not need to be adjusted for PBS for typical downstream applications.
Przejdź do
N9284
Nitroreductase from Escherichia coli
Bardziej ekologiczna alternatywa
≥90% (SDS-PAGE), recombinant, expressed in E. coli
Synonim(y):
NTRA
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
1 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1710,00 zł |
Informacje o tej pozycji
Specific activity:
≥100 units/mL
Assay:
≥90% (SDS-PAGE)
Recombinant:
expressed in E. coli
1710,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócrecombinant
expressed in E. coli
Quality Segment
assay
≥90% (SDS-PAGE)
form
lyophilized powder
specific activity
≥100 units/mL
mol wt
monomer 24000
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UniProt accession no.
greener alternative category
shipped in
wet ice
storage temp.
−20°C
Gene Information
Escherichia coli K12 ... nfsB(945483)
General description
Nitroreductase is a flavoprotein and is encoded by the NfsB gene. It comprises a dimer, with 217 amino acids and active site in each subunit. The structure has FMN and the substrate bound to the enzyme.
Lyophilized powder containing PBS. Does not contain BSA as excipient
Application
Nitroreductase from Escherichia coli has been used in the conjugation generation with pig liver esterase (PLE). It has also been used in chemiluminescence response studies with probes HyCL-3 and HyCL-4-AM in rat liver microsomes.
Biochem/physiol Actions
Nitroreductase (NTR) catalyzes the reduction of nitroaromatic substrates and quinones. The mutant F124K of NTR is useful in cancer therapy and improves sensitization of drug CB1954.
Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical.
Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.
Preparation Note
Produced using animal component-free materials.
Other Notes
One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C.
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Ta pozycja | |||
|---|---|---|---|
| description ≥90% (SDS-PAGE), recombinant, expressed in E. coli | description ammonium sulfate suspension, >25 units/mg protein (Bradford) | description recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein | description lyophilized powder, 400-2,000 units/mg protein |
| Gene Information Escherichia coli K12 ... nfsB(945483) | Gene Information Escherichia coli K12 ... trxB(949054) | Gene Information Escherichia coli K12 ... trxA(948289) | Gene Information Escherichia coli K12 ... glnA(948370) |
| specific activity ≥100 units/mL | specific activity >25 units/mg protein (Bradford) | specific activity ≥3 units/mg protein | specific activity 400-2,000 units/mg protein |
| assay ≥90% (SDS-PAGE) | assay - | assay ≥90% (SDS-PAGE) | assay - |
| recombinant expressed in E. coli | recombinant - | recombinant expressed in E. coli | recombinant - |
| form lyophilized powder | form ammonium sulfate suspension | form essentially salt-free, lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
Klasa składowania
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Can I dissolve the 1 mg in 10 mL water to make aliquots. Does it matter the concentration of the aliquots due to the presence of specific amount of PBS
1 answer-
Helpful?
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I want to dissolve this 1 mg solid and make it into 100 aliquots for long term storage. Could you tell me what buffer I should use it to dissolve it?
1 answer-
Each vial contains 1 mg of Nitroreductase, 20 mg lactose, and 10 mg of PBS buffer salts. The vial can be reconstituted in water.
Helpful?
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