N1290

Polynucleotide phosphorylase human

• Synonim(y): Polyribonucleotide nucleotidyltransferase
• UNSPSC Code: 12352204
• NACRES: NA.54
• Numer WE: 2.7.7.8 (BRENDA, IUBMB)
• MDL number: MFCD00131992
• Recombinant: expressed in E. coli
• Concentration: 400-600 μg/mL protein

Właściwości

• recombinant: expressed in E. coli
• form: solution
• enzyme activity: ≥20 units/mg protein
• mol wt: ~90 kDa
• concentration: 400-600 μg/mL protein
• shipped in: dry ice
• storage temp.: −70°C

Opis

Biochem/physiol Actions

Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3′ to 5′ exoribonuclease activity and a 3′-terminal oligonucleotide polymerase activity. It is also involved in mRNA processing and degradation in bacteria, plants, and humans.

Physical form

supplied as a solution in 20 mM HEPES buffer, pH 7.9, with 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl2, ~130 mM KCl, and 20% (w/v) glycerol.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• Co się dzieje?: WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable