MTOX1001

MDR1 Knockout Caco-2 Cells

human male colorectal tissue (Source Disease: colon adenocarcinoma)

• Synonim(y): C2BBe1 Cells MDR1 (-/-/-/-)
• UNSPSC Code: 41106514
• NACRES: NA.81
• Biological source: human male colorectal tissue (Source disease: colon adenocarcinoma)

Właściwości

• Nazwa produktu: MDR1 Knockout Caco-2 Cells, one vial
• biological source: human male colorectal tissue (Source disease: colon adenocarcinoma)
• Quality Segment: 200
• form: liquid
• technique(s): drug transporter assay: suitable, permeability assay: suitable
• OMIM accession no.: 171050
• application(s): ADME/TOX
• storage temp.: −196°C
• Gene Information: human ... ABCB1(5243)

Opis

General description

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MDR1 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCB1 (MDR1) efflux transporter.

Application

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

Features and Benefits

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:

• A functional knockout of the MDR1 gene eliminates the reliance on chemical inhibitors to determine if a compound is an MDR1 substrate
• The 24 well Transwell format enables the MDR1 knockout cells to be included in standard drug transporter protocols
• Human assay with no interference from animal inhibitors
• Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

Legal Information

ADME/Tox Cell Lines License

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Elementy zestawu są też dostępne oddzielnie

• MTOX1001

  MDR1 Knockout Caco-2 Cells, one vial 1 vial

  Karta charakterystyki

Informacja o środkach bezpieczeństwa

• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Klasa składowania: 12 - Non Combustible Liquids