Starting with total RNA or samples pre-enriched or microRNAs, the MystiCq® microRNA cDNA Synthesis Mix kit contains all the components necessary to convert mature microRNAs into cDNA templates for qPCR.
MIRRT
MystiCq® microRNA cDNA Synthesis Mix
Synonim(y):
Mieszanina do syntezy cDNA mikroRNA
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352200
NACRES:
NA.55
Aby zapytać o ten produkt MIRRT, skontaktuj się z lokalnym biurem lub sprzedawcą firmy Merck. Skontaktuj się z zespołem ds. pomocy technicznej
Polecane produkty
metody
cDNA synthesis: suitable
Warunki transportu
wet ice
temp. przechowywania
−20°C
Opis ogólny
Starting with total RNA or samples pre-enriched or microRNAs, the MystiCq® microRNA cDNA Synthesis Mix kit contains all the components necessary to convert mature microRNAs into cDNA templates for qPCR. Since microRNAs are not naturally polyadenylated, the first step in the cDNA synthesis process is to polyadenylate the microRNAs through a poly(A) polymerase reaction. Next, the ReadyScript reverse transcriptase, an oligo-dT adapter primer and other required reagents are added to convert the polyadenylated microRNAs into cDNA. The adapter primer includes a unique sequence at the 5′ end that is complementary to the sequence of the MystiCq Universal PCR Primer, another component of the MystiCq microRNA qPCR Assay System.The kit also contains a Positive Control Primer that is specific to SNORD44, a small nucleolar RNA that is widely expressed in most human tissues. Sufficient amounts of the Poly(A) Tailing Buffer and the MicroRNA cDNA Reaction mix are included in the kit to allow for the use of no poly(a) polymerase and no reverse transcriptase control reactions. To analyze individual microRNAs, use the MystiCq Universal PCR Primer (which targets the adapter primer sequence) together with the MystiCq microRNA qPCR Assay Primer and the MystiCq® microRNA SYBR® Green qPCR ReadyMix™.
Zastosowanie
Cechy i korzyści
- Separate polyadenylation and reverse transcription steps allow for the possibility of optimization of protocol if necessary
- cDNA produced may be archived for future use
- Single RT reaction can provide up to 1000 qPCR reactions
- Includes a positive control primer for use with most human tissues
Opakowanie
Default reaction volume is 20 μL
Informacje prawne
MystiCq is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
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Tylko elementy zestawu
Numer produktu
Opis
- Poly (A) Tailing Buffer (5X)
- Human Positive Control Primer
- Nuclease-free Water
- MystiCq® Universal PCR Primer
- Poly (A) Polymerase
- microRNA cDNA Reaction Mix
- ReadyScript® Reverse Transcriptase
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Kod klasy składowania
10 - Combustible liquids
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PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
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I want to work with the cDNA of mARN, can I synthetize the cDNA of the mARN starting from a Total ARN sample using this kit? Or does the kit only synthetize the cDNA from the microARN?
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