While not strictly necessary, running samples in duplicate is strongly recommended to assess result consistency. If a sample yields unexpected results, duplicate measurements help determine whether the result is an isolated measurement or reproducible. For pilot or screening tests aimed at assessing sample compatibility or dilution ranges, single runs are acceptable, as these are not intended as final results.
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MAK315
Oxalate Assay Kit
sufficient for 100 colorimetric tests
Synonim(y):
Oxalic Acid Detection Kit
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 kit | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2870,00 zł |
Informacje o tej pozycji
NACRES:
NA.84
UNSPSC Code:
12161503
2870,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócdetection method
colorimetric
relevant disease(s)
genitourinary disorders
storage temp.
−20°C
General description
Oxalate or Oxalic Acid is a metabolic breakdown product of the Kreb′s Cycle in eukaryotes, and the glyoxylate cycle in other microorganisms. It can be found in the urine of humans and other mammals. Oxalate concentration can be used as a measure of kidney function where a high level of oxalate is an indicator for kidney stones which are primarily made of the insoluble salt calcium oxalate. Measuring oxalate is more accurate than measuring calcium as a marker for kidney stones because calcium is excreted at high concentrations even in normal urine.
Application
Suitable for the quantitative determination of oxalate (oxalic acid) in urine, animal and plant tissue samples.
Biochem/physiol Actions
Simple, direct and high-throughput assays for measuring oxalate concentration find wide applications. This oxalate assay kit uses a single Working Reagent that combines the oxalate oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 595 nm is directly proportional to oxalate in the sample.
Features and Benefits
Compatible with high-throughput handling systems.
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Ta pozycja | ||
|---|---|---|
| description sufficient for 100 colorimetric tests | description sufficient for 100 colorimetric tests | description reference material for titrimetry, certified by BAM, >99.5% |
| detection method colorimetric | detection method colorimetric | detection method - |
| storage temp. −20°C | storage temp. −20°C | storage temp. - |
| relevant disease(s) genitourinary disorders | relevant disease(s) gastrointestinal diseases | relevant disease(s) - |
Klasa składowania
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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Running in duplicate means you only get 16 samples per 96 well plate (6 wells per sample are used). Each kit only does 100 tests so largely one 96 well plate. How necessary is it to run all in duplicate?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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According to the instructions, is it supposed to add 10 uL of sample (or diluted sample) to each of the 3 wells (blank, sample and sample standard)? If so, why you then add 10 uL of water/standard to the respective well, and nothing to the sample well?
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10 µL of sample is added to each of the three wells (blank, sample, and internal standard). Following that step, 10 µL of ultrapure water is added to the sample blank and sample wells, while 10 µL of standard is added to the internal standard well. This ensures that the volumes are equivalent across the wells.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What is the purpose of acidifying the samples for longer-term storage?
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Acidification of urine samples is necessary for longer-term storage. Oxalate demonstrates greater solubility under acidic conditions. Additionally, ascorbic acid (Vitamin C) in urine samples rapidly degrades into oxalate in non-acidified samples, so acidifying urine should help to prevent this. Given that the Working Reagent used in this assay is acidic, acidifying fresh samples prior to the assay should not be necessary.
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What is the approximate expiration time for these kits?
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Expiration dating is batch specific and should be determined from the lot specific Certificate of Analysis. Please review your batch specific Certificate of Analysis by searching your lot number in the DOCUMENTATION section of the Product Detail Page:
https://www.sigmaaldrich.com/product/sigma/MAK315#product-documentationHelpful?
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