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MAK242

Thrombin Activity Fluorometric Assay Kit

sufficient for 100 fluorometric tests

Synonim(y):

Thrombin Enzyme Activity Kit

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Informacje o tej pozycji

NACRES:
NA.84
UNSPSC Code:
12161503

detection method

fluorometric

relevant disease(s)

hematological disorder

storage temp.

−20°C

General description

Thrombin enzyme (Factor IIa, EC 3.4.21.5), a Serine protease, is an important clotting factor in the coagulation cascade that involves the conversion of soluble fibrinogen to insoluble active fibrin strands. In this pathway, Prothrombin is proteolytically converted into an active Thrombin.[1]

Application

Suitable for determining the activity of pure Thrombin and for detecting the activity of Thrombin in plasma.

Biochem/physiol Actions

Thrombin activity fluorometric assay kit utilizes the ability of thrombin to proteolytically cleave a synthetic substrate and release a fluorophore, 7-amino-4-methylcoumarin (AMC), which can be easily quantified by fluorescence. This assay kit is simple, rapid and can detect thrombin activity as low as 1ng in samples.
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Klasa składowania

10 - Combustible liquids

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
7H26K03730
7B24K03730
SLCC7286

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Powiązane treści

The conversion of prothrombin to thrombin I. Characterization of the reaction products formed during the activation of bovine prothrombin.
Owen W G, et al.
The Journal of Biological Chemistry, 249(2), 594-605 (1974)
Crystal structure of the thrombin-hirudin complex: a novel mode of serine protease inhibition.
Grutter M G, et al.
The Embo Journal, 9(8), 2361-2365 (1990)

Numer pozycji handlu globalnego

SKUNUMER GTIN
MAK242-1KT04061833318546

Questions

  1. Hey just wondering how should we detect the AMC fluorophore released by the substrate? We have flow cytometer in the lab, but not sure how we can detect it.

    1 answer

    1. Please review the protocol at the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/176/154/mak242bul.pdf

      This kit is not intended for use in flow cytometry. A typical measurement is done on a plate reader like those presented in the link below:
      https://www.sigmaaldrich.com/substance/benchmarksmartreader96microplateabsorbancereaderwithincubation1234598765

      To discuss further, please navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Product Technical Inquiries" under the Products Section with all the required information so that a member of the Technical Service team can reach out to assist further. Thank you.

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