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MAK222

Beta-Lactamase Inhibitor Screening Kit

Sufficient for 100 Colorimetric tests

Synonim(y):

β-Lactamase Inhibitor Screening Kit

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Informacje o tej pozycji

NACRES:
NA.84
UNSPSC Code:
12161503

detection method

colorimetric

storage temp.

−20°C

General description

β-Lactamase (β-lactamase, βL, EC 3.5.2.6) is an enzyme first identified in Escherichia coli and has been described as penicillinase. A number of βLs have since been identified from various bacteria. β-Lactamases specifically hydrolyze β-lactam rings present in antibiotics such as penicillin, cephalosporins, monobactam, and carbapenem, and confer resistance against these antibiotics.

Application

Beta-Lactamase Inhibitor Screening Kit has been used in enzymatic assays.
Suitable for the screening of inhibitors of β-Lactamase

Biochem/physiol Actions

The β-Lactamase Inhibitor Screening Kit is a rapid, simple and sensitive assay that is suitable for high throughput screening of β-Lactamase inhibitors. βL activity is measured by hydrolyzing a chromogenic cephalosporin called nitrocefin, producing a colorimetric product (A490), proportional to the enzymatic activity present.

Features and Benefits

Compatible with high-throughput handling systems.
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Tylko elementy zestawu

Numer produktu
Opis
  • β-Lactamase Assay Buffer
  • Nitrocefin, in DMSO
  • β-Lactamase
  • Inhibitor Control, Clavulanic acid

pictograms

Health hazard
GHS08

signalword

Danger

Klasa składowania

10 - Combustible liquids

Hazard Classifications

ED ENV 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  

  1. What is the concentration of clavulanic acid?

    1 answer

    1. This product is discontinued. However, the original stock concentration in the inhibitor component is 100 mM Clavulanic Acid.

      Helpful?

  2. Hello, I want to use your β-lactamase inhibitor screening kit, however I could not find which β-lactamase(s) the kit includes, and where I can a step by step protocol

    1 answer

    1. Here is a copy of the protocol for this product: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/767/737/mak222pis-ms.pdf

      Included are nitrocefin, β-lactamase, a β-lactamase assay buffer, and the inhibitor control clavulanic acid. These are not sold as distinct products on the website.

      Helpful?

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