The protocol is included in the product datasheet for this item. Please see the link below to review this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/270/922/mak196bul.pdf
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MAK196
Malate Dehydrogenase Assay Kit
Sufficient for 100 Colorimetric tests
Synonim(y):
MDH Activity Assay Kit
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Informacje o tej pozycji
NACRES:
NA.84
UNSPSC Code:
12161503
detection method
colorimetric
relevant disease(s)
neurological disorders
storage temp.
−20°C
Gene Information
human ... MDH1(4190), MDH1B(130752), MDH2(4191)
mouse ... MDH1(17449), MDH1B(76668), MDH2(17448)
rat ... MDH1(24551), MDH1B(316444), MDH2(81829)
General description
Malate dehydrogenase (MDH; EC 1.1.1.37) is an NAD oxidoreductase that catalyzes the NAD/NADH-dependent interconversion of malate and oxalacetate. This reaction is critical to maintain the malate/aspartate shuttle across the mitochondrial membrane and Krebs tricarboxylic acid cycle in the mitochondrial matrix. Increased MDH activity has been observed in neurodegenerative diseases such as Alzheimer′s disease.
Application
Suitable for the detection of Malate Dehydrogenase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.
Biochem/physiol Actions
The Malate Dehydrogenase Assay Kit provides a simple and sensitive procedure for measuring MDH activity in a variety of tissues, cell cultures, and isolated mitochondria. MDH activity is determined by generating a product with absorbance at 450 nm proportional to the enzymatic activity present. One unit of MDH is the amount of enzyme that generates 1.0 μmole of NADH per minute at 37 °C and pH 9.5.
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Ta pozycja | |||
|---|---|---|---|
| Gene Information human ... MDH1(4190), MDH1B(130752), MDH2(4191) | Gene Information human ... LDHA(3939), LDHB(3945) | Gene Information human ... IDH1(3417), IDH2(3418), IDH3B(3420), IDH3G(3421) | Gene Information human ... GOT1(2805) |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method colorimetric |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| relevant disease(s) neurological disorders | relevant disease(s) hematological disorder; cancer | relevant disease(s) cancer | relevant disease(s) gastrointestinal diseases; cardiovascular diseases |
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Tylko elementy zestawu
Numer produktu
Opis
- MDH Assay Buffer
- MDH Substrate
- MDH Enzyme Mix
- MDH Developer
- NADH Standard
- MDH Positive Control
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Klasa składowania
8A - Combustible corrosive hazardous materials
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Is there a protocol associated with the kit? This general protocol doesn't use MDH developer: https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/_enzymatic-assay-of-malic-dehydrogenase
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