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MAK137

Sigma-Aldrich

Phospholipase D Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonim(y):

PLD Activity Assay Kit

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About This Item

Kod UNSPSC:
12161503
NACRES:
NA.84

zastosowanie

sufficient for 100 colorimetric or fluorometric tests

metoda wykrywania

colorimetric
fluorometric

powiązane choroby

cancer

temp. przechowywania

−20°C

informacje o genach

human ... PLD1(5337) , PLD2(5338) , PLD3(23646) , PLD4(122618) , PLD5(200150) , PLD6(201164)
mouse ... PLD1(18805) , PLD2(18806) , PLD3(18807) , PLD4(104759) , PLD5(319455) , PLD6(194908)
rat ... PLD1(25096) , PLD2(25097) , PLD3(361527) , PLD4(362792) , PLD5(289270) , PLD6(287366)

Powiązane kategorie

Opis ogólny

Phospholipase D (PLD) catalyzes the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and choline. It is ubiquitously expressed and is implicated in immune function and cancer inhibition. Abnormalities in PLD expression have been associated with human cancers such as breast, renal, gastric and colorectal cancer.

Zastosowanie

Phospholipase D (PLD) Activity Assay Kit has been used to measure PLD activity in the liver and lysosomal fractions of the mice liver.
Suitable for the detection of phospholipase D activity in biological samples. This kit can be used to study the effects of various compounds on phospholipase D activity.

Cechy i korzyści

Compatible with high-throughput handling systems.

Przydatność

Suitable for the quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.

Zasada

In this assay, PLD hydrolyzes phosphatidylcholine to choline, which is determined using choline oxidase resulting in a colorimetric (570 nm)/fluorometric (λex = 530/λem = 585 nm) product, proportional to the PLD activity in the sample. Unit definition: one unit of PLD catalyzes the formation of 1 mmole choline per minute under the assay conditions (pH 7.4).

Piktogramy

Health hazard
GHS08

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H334,H412

Zwroty wskazujące środki ostrożności

P261 - P273 - P284 - P501

Klasyfikacja zagrożeń

Aquatic Chronic 3 - Resp. Sens. 1

Kod klasy składowania

10 - Combustible liquids

Temperatura zapłonu (°F)

188.6 °F - closed cup

Temperatura zapłonu (°C)

87 °C - closed cup

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Walid El Kayal et al.
Horticulture research, 4, 17042-17042 (2017-11-09)
Raspberry (Rubus spp.) is an economically important crop with a restricted growing season and very limited fruit shelf-life due to its extreme tenderness. In order to prolong its shelf life, an aqueous composition containing hexanal as the key active ingredient
Phospholipase D.
Su W and Michael A F
Handbook of Cell Signaling, 1167-1176 (2009)
Karthika Sriskantharajah et al.
Plants (Basel, Switzerland), 10(11) (2021-11-28)
'Honeycrisp' (Malus domestica Borkh.), a premium applecultivar, is highly susceptible to bitter pit and decline in quality during long-term storage. In order to enhance the quality, an aqueous composition containing hexanal was applied as a preharvest spray. The effects of
Przemysław Bernat et al.
Journal of plant physiology, 229, 158-163 (2018-08-11)
2,4-dichlorophenoxyacetic acid (2,4-D) is among the most commonly used herbicides applied for weed control during wheat cultivation. However, its application could affect wheat growth. The present study investigates the effect of the ascomycetous fungus Trichoderma harzianum on lipid peroxidation, phospholipids
Anne Lise Ferrara et al.
Journal of immunology research, 2019, 5836476-5836476 (2019-07-30)
Mastocytosis is a condition characterized by the expansion and accumulation of mast cells (MCs) in various organs. The symptoms are related to the increased release of MC-derived mediators that exert local and distant effects. MCs are a source and target
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