L-Carnitine in solution appears to be relatively stable when stored at –20 °C. This kit includes an L-Carnitine standard. When reconstituted, this standard may be stored at –20 °C for 3 months. Serum samples would presumably have similar stability at –20 °C. Serum products that are offered for culture or similar uses, which are stored at –20 °C, have a general shelf life of 3 - 4 years.
See the link below to review the product datasheet for this kit:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/353/360/mak063bul.pdf
Przejdź do
MAK063
L-Carnitine Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonim(y):
Carnitine Quantification Kit
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Informacje o tej pozycji
NACRES:
NA.84
UNSPSC Code:
12161503
usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric, fluorometric
storage temp.
−20°C
General description
Carnitine is a metabolite that is synthesized from lysine and methionine. Carnitine is essential for fatty acid β-oxidation due to its role in fatty acid transport into the mitochondrial matrix via the carnitine/acylcarnitine shuttle. Carnitine exists in two stereoisomers but only the L-carnitine isomer is biologically active. Tissue levels of L-carnitine decrease with aging and decreased L-carnitine may contribute to age-related mitochondrial decline. The L-Carnitine Assay Kit is a simple convenient means of measuring free L-Carnitine in biological samples such as serum.
Application
Suitable for the measurement of free L-Carnitine in biological samples such as tissue, cells and serum
L-Carnitine Assay Kit has been used to determine the concentration of carnitine in serum samples.
Biochem/physiol Actions
L-carnitine concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the L-carnitine present. Typical sensitivities of detection for this kit are 10 μM (colorimetric) and 1 μM (fluorometric). The normal range for serum L-Carnitine is between 10-70 μM.
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Klasa składowania
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
wgk
WGK 3
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for how long can plasma or serum samples be frozen?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can these kits be used with serum samples from mouse?
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Yes, this kit can be used on mouse serum samples. We generally recommend that fresh serum samples be used. Frozen samples can probably be used. However, the successful use of frozen samples depends on the efficiency of freezing them and the amount of time they have been frozen. If previously frozen serum samples are used, you may want to check the performance of the kit with fresh vs. frozen samples.
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