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Merck

M7292

M16 Medium

With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered

Synonim(y):

Cell Culture Medium, Cell Culture Medium M16, M16 Medium Solution

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Wybierz wielkość

50 ML

359,00 zł

100 ML

600,00 zł

359,00 zł


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Informacje o tej pozycji

NACRES:
NA.75
UNSPSC Code:
12352207

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Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | embryo: suitable

impurities

endotoxin, tested

components

NaHCO3: 2.101 g/L
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
glucose: 1.0 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

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Ta pozycja
M716714361CR5158
form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | embryo: suitable

technique(s)

cell culture | embryo: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

components

NaHCO3: 2.101 g/L
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
glucose: 1.0 g/L (Dextro)

components

HEPES: 5.42726 g/L
NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L

components

NaHCO3: 2.10 g/L
phenol red: no
HEPES: no
glucose: 5.6 g/L

components

phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
NaHCO3: 2 g/L
HEPES: 5.96 g/L
glucose: 2 g/L (Dextro)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Application

M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.

Preparation Note

Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.

Analysis Note

Presence of particulates in solution will not affect product′s performance.
This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.
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Klasa składowania

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Qian-Qian Sha et al.
Nature communications, 9(1), 3477-3477 (2018-08-30)
Trimethylation of histone H3 on lysine-4 (H3K4me3) is associated with gene-regulatory elements, but its transcription-independent function in cell division is unclear. CxxC-finger protein-1 (CFP1) is a major mediator of H3K4 trimethylation in mouse oocytes. Here we report that oocyte-specific knockout
Marco Ragusa et al.
RNA biology, 16(9), 1237-1248 (2019-05-29)
Circular RNAs (circRNAs) have a critical role in the control of gene expression. Their function in spermatozoa (SPZ) is unknown to date. Twenty-eight genes, involved in SPZ/testicular and epididymal physiology, were given in circBase database to find which of them
Hong-Bin Liu et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 6(15), 1900295-1900295 (2019-08-14)
A number of genes involved in zygotic genome activation (ZGA) have been identified, but the RNA-binding maternal factors that are directly related to ZGA in mice remain unclear. The present study shows that maternal deletion of Igf  2bp2 (also commonly known
Yi-Wen Xu et al.
Journal of cell science, 130(19), 3297-3307 (2017-08-19)
Precise regulation of DNA replication and genome integrity is crucial for gametogenesis and early embryogenesis. Cullin ring-finger ubiquitin ligase 4 (CRL4) has multiple functions in the maintenance of germ cell survival, oocyte meiotic maturation, and maternal-zygotic transition in mammals. DDB1-cullin-4-associated
Lan-Rui Cao et al.
Frontiers in cell and developmental biology, 8, 609430-609430 (2020-12-08)
In mammalian species, both the maturation promoting factor (MPF) and the mitogen-activated protein kinase (MAPK) cascade play critical roles in modulating oocyte meiotic cell-cycle progression. MPF is a critical heterodimer composed of CDK1 and cyclin B1. Activation of MPF and

Produkty

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

Numer pozycji handlu globalnego

SKUNUMER GTIN
M7292-100ML04061834061854
M7292-50ML04061834061878

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