Rekombinowany marker masy cząsteczkowej został użyty do określenia wielkości pasm białkowych w Western blot i elektroforezie w żelu poliakryloamidowym z dodecylosiarczanem sodu (SDS-PAGE).[1][2]
Inne uwagi
The recombinant molecular weight standard for SDS-PAGE contains seven precisely sized proteins, M.W. 15, 25, 35, 50, 75, 100 and 150 kDa. Recombinant proteins produce sharp bands for precise size determination since they are not glycosylated.
Postać fizyczna
Solution containing approx. 0.8 mg/ml total protein in 125 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 200 mM 2-mercaptoethanol, 0.007% bromphenol blue.
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Earlier studies have demonstrated in A/Sn mice highly susceptible to Chagas' disease protective immunity against lethal Trypanosoma cruzi infection elicited by vaccination with an open reading frame (ORF) expressed by amastigotes. In our experiments, we used this mouse model to
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