L8525

Lipase from Candida rugosa

lyophilized powder, ≥40,000 units/mg protein

• Synonim(y): Triacylglycerol acylhydrolase, Triacylglycerol lipase
• Numer CAS: 9001-62-1
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-619-9
• Numer WE: 3.1.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00131509
• Specific activity: ≥40,000 units/mg protein
• Biological source: fungus (Candida rugosa)

Właściwości

• biological source: fungus (Candida rugosa)
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥40,000 units/mg protein
• composition: Protein, ≥4.0%
• foreign activity: α-amylase ≤0.05 unit/mg solid, protease ≤0.001 units/mg solid
• storage temp.: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChI key: QWZUIMCIEOCSJF-CHHCPSLASA-N

Opis

General description

Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.

Application

Lipase from C. rugosa is used to catalyze the synthesis of flavor esters such as pentyl propanoate and butyl butanoate used in food enhancement.

Lipase from Candida rugosa has been used:

• in the hydrolysis of loaded polycaprolactone–20% tricalcium phosphate (PCL–TCP) composites
• in adsorption of proteins in emulsan/alginate beads and in enzyme assays
• to catalyse methanolysis and hydrolysis reactions

Lipase from Candida rugosa has been used in a study to assess the influence of phase segregation on enzyme hydrolytic activity.

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Lipases are used in detergent, food, leather, pharmaceutical and diary industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.2 at 37 °C using olive oil (30 minute incubation).

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)