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L8146
Lectin from Ulex europaeus (gorse, furze)
peroxidase conjugate, lyophilized powder
Lectin from Ulex europaeus (gorse, furze)
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Synonim(y):
Ulex europaeus agglutinin, UEA-I
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
0.5 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1340,00 zł |
Informacje o tej pozycji
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
Assay:
70.00-90.00% (Lowry)
Biological source:
Ulex europaeus
1340,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
Ulex europaeus
Quality Segment
conjugate
peroxidase conjugate
assay
70.00-90.00% (Lowry)
form
lyophilized powder
potency
<40 μg/mL agglutination activity
peroxidase activity
≥15 units/mg protein
composition
Protein, ~80% Lowry
technique(s)
western blot: suitable
storage temp.
2-8°C
Application
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Biochem/physiol Actions
Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L -fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.
Packaging
Package size based on protein content.
Physical form
Contains sodium citrate buffer salts
Preparation Note
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.
Other Notes
Conjugates are prepared from affinity purified lectin.
One unit will form 1.0 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.
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Ta pozycja | |||
|---|---|---|---|
| description peroxidase conjugate, lyophilized powder | description biotin conjugate, lyophilized powder | description Leucoagglutinin PHA-L, lyophilized powder, BioReagent, suitable for cell culture | description BioReagent, suitable for cell culture, lyophilized powder |
| assay 70.00-90.00% (Lowry) | assay - | assay - | assay - |
| technique(s) western blot: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| biological source Ulex europaeus | biological source - | biological source - | biological source - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| potency <40 μg/mL agglutination activity | potency <50 μg/mL agglutination activity | potency erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at 250 μg lectin/ml.), ≤1.0 μg per mL leucoagglutination activity, ≤5 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells) | potency - |
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Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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