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L6135

Lectin from Arachis hypogaea (peanut)

biotin conjugate, lyophilized powder

Synonim(y):

PNA, Peanut agglutinin

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Informacje o tej pozycji

NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
Mol wt:
120 kDa


conjugate

biotin conjugate

Quality Segment

form

lyophilized powder

mol wt

120 kDa

composition

Protein, ≥80% Lowry

extent of labeling

~3 mol biotin per mol protein

technique(s)

blood typing: suitable, fractionation: suitable

solubility

water: 1.00-1.10 mg/mL, clear to slightly hazy, colorless

storage temp.

2-8°C

General description

Peanut lectin (PNA) is a lectin or an agglutinin from the legume, Arachis hypogaea, found in peripheral venous blood in humans after ingestion of peanuts. PNA lectin is specific to the galactosyl (β-1,3) N-acetylgalactosamine.

Application

Lectin from Arachis hypogaea (peanut) has been used:
  • in enzyme-linked micro plate assay to detect β-D-galactose-N-acetylglucosamine
  • in Immunohistochemical double staining
  • Immunohistochemical double staining for the detection of germinal centers
  • in enzyme-linked microplate assayto detect β-D-galactose-N-acetylglucosamine

Biochem/physiol Actions

PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.
Lectin from Arachis hypogaea (peanut) functions as an antigenic determinant for Thomsen-Friedenreich antigen (TF) and as mitogen for colorectal cancer stimulation cell division in normal colonic epithelium. It is therefore used as tumor marker.

Packaging

Package size based on protein content.

Physical form

Contains sodium citrate
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Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Certyfikaty analizy (CoA)

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Questions

1–3 of 3 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. How does the storage temperature relate to shipping conditions?

    1 answer
    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  3. Is the peanut lectin (Lectin from Arachis hypogaea) a glycoprotein?  If so, what is the carbohydrate content?

    1 answer
    1. Peanut lectin is not a glycoprotein. Although peanut lectin binds specifically to a particular carbohydrate (beta-gal(1-->3)galNac) in other molecules, the lectin itself does not contain any carbohydrate.  Please see page 231 of the following paper: R. Banerjee, et al., "Crystal structure of peanut lectin, a protein with an unusual quaternary structure".  Proc. Nat. Acad. Sci., USA, 91, 227-231 (1994).

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