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L3038

Lysenin from Eisenia foetida

solid

Synonim(y):

Eisenia Lysenin, Lysenin Protein

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Informacje o tej pozycji

UNSPSC Code:
12352200
NACRES:
NA.32
MDL number:
MFCD02687941

form

solid

Quality Segment

200

mol wt

33 kDa

concentration

≥50% (SDS-PAGE)

storage temp.

−20°C

General description

Lysenin is a superfamily of certain proteins including lysenin-related protein 1 (LRP-1, lysenin 2) and LRP-2 (lysenin 3).

Application

Lysenin from Eisenia foetida has been used to treat B cells and study lysenin′s effect on membrane diacylglycerol (DAG) and surface sphingomyelin (SM).[1]

Biochem/physiol Actions

Lysenin is a 33kDa protein present in the coelomic fluid of the earthworm Eisenia foetida. It interacts with sphingomyelin in cell membranes. In vertebrates, this binding results in cytotoxicity and contraction of smooth muscle in vitro as well as vasodepressor activity and lethality under in vivo conditions.[2]
Lysenin serves as a tool to explore membrane lipid organization.[3] It is known to induce hemolysis in vertebrates and mammalian cells.
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Ta pozycja
S1135C4063SRP3200
Lysenin from Eisenia foetida solid

Sigma-Aldrich

L3038

Lysenin from Eisenia foetida

Stroma from bovine erythrocytes source of stromal-bound enzymes

Sigma-Aldrich

S1135

Stroma from bovine erythrocytes

C Reactive Protein from human fluids buffered aqueous solution

Sigma-Aldrich

C4063

C Reactive Protein from human fluids

GDNF from mouse recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Sigma-Aldrich

SRP3200

GDNF from mouse

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

-

form

solid

form

solid

form

buffered aqueous solution

form

lyophilized

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

mol wt

33 kDa

mol wt

-

mol wt

-

mol wt

15.1 kDa

concentration

≥50% (SDS-PAGE)

concentration

-

concentration

-

concentration

-

Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

  1. What's the solubility of the Lysenin samples in water? I desovate the 50ug sample in 0.6 mL water, looks a lot of not solutable? How to you usaually desovate the samples? In water or any buffers? I am woring on Lysenin reconstitution in to lipid bilyers.

    1 answer

    1. The solubility for the product is not analyzed on a lot to lot basis. However, it has been cited that solution were prepared by dissolving lysenin (1 mg/ml in 2% BSA-PBS) or 50 μg of lysenin in 50 μl of 10 mM HEPES/KOH (pH 7.0) containing 30 mM KCl and 50% Glycerol.

      Kindly review the following citations:
      Yamaji A, Sekizawa Y, Emoto K, Sakuraba H, Inoue K, Kobayashi H, Umeda M. Lysenin, a novel sphingomyelin-specific binding protein. J Biol Chem. 1998 Feb 27;273(9):5300-6. doi: 10.1074/jbc.273.9.5300. PMID: 9478988.

      Faouri RA, Krueger E, Govind Kumar V, Fologea D, Straub D, Alismail H, Alfaori Q, Kight A, Ray J, Henry R, Moradi M, Salamo G. An Effective Electric Dipole Model for Voltage-induced Gating Mechanism of Lysenin. Sci Rep. 2019 Aug 7;9(1):11440. doi: 10.1038/s41598-019-47725-0. PMID: 31391571; PMCID: PMC6686002.

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