I8907

Interferon β from rat

≥95%, recombinant, expressed in CHO cells, lyophilized powder, suitable for cell culture

• Synonym(s): IFN-β
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD04040885

Properties

• biological source: rat
• Quality Segment: 200
• recombinant: expressed in CHO cells
• assay: ≥95%
• form: lyophilized powder
• mol wt: ~20 kDa
• packaging: pkg of 1 vial
• concentration: ≥100000 units/mL
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: P70499
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: rat ... Ifnb1(24481)

Description

Biochem/physiol Actions

Cytokine with anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.

Interferon β belongs to the type 1 interferon family pf proteins.[1] It is also needed for the inhibition of tumor cell growth via cell death, S-phase increase and mediating differentiation.[2] It is also important for bone homeostasis.[3]

Physical form

Lyophilized from phosphate buffered saline (PBS) containing 125 mM trehalose.

Analysis Note

The biological activity is determined in the cytopathic effect inhibition assay using rat epithelial-like (RATEC) cells with vesicular stomatitis virus (VSV). The cytopathic effect of 50% is produced with approximately 1 unit/ml interferon. Since there is no international rat interferon β standard, units are determined with respect to the NIH international standard reference for mouse interferon α/β.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)