I3659

Monoclonal Anti-ING1 antibody produced in mouse

~2 mg/mL, clone Cab 3, purified immunoglobulin, buffered aqueous solution

• Synonim(y): Anti-Inhibitor of Growth 1, Anti-p33ING1
• UNSPSC Code: 12352203
• MDL number: MFCD03455695
• Conjugate: unconjugated
• Clone: Cab 3, monoclonal
• Application: ARR, ELISA (i), ICC, WB
• Citations: 6

Właściwości

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: Cab 3, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~33 kDa
• species reactivity: human
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 2-4 μg/mL using K562 total cell extract
• isotype: IgG1
• UniProt accession no.: Q9UK53
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... ING1(3621)

Opis

General description

Three alternatively spliced mRNAs of the gene ING1 (inhibitor of growth family member 1), p47ING1a, p33ING1b and p24ING1c, encode for proteins that function as growth inhibitors. The proteins are localized to the nucleus and they have a common C-terminal region. The major isoforms expressed in humans are p47ING1a and p33ING1b. The gene ING1 is mapped to human chromosome 13q34.

Immunogen

recombinant ING1.

Biochem/physiol Actions

The ING (inhibitor of growth) family of proteins is involved in several cellular processes, such as cell cycle, senescence and apoptosis. They activate acetylation-dependent pathways that in turn, regulate gene expression. The isoform p33ING1b is found to be involved in the regulation of apoptosis. Suppression of p33ING1b expression is found to promote the formation of tumor. On the other hand, increased expression arrests cells at G1 phase of the cell cycle and inhibits the progression of cell cycle. These processes are mediated by interactions with p53 tumor suppressor. Human ING1 proteins are found to be associated with proteins involved in histone acetyltransferase (HAT) activity, such as TRRAP (transformation/transcription domain associated protein), PCAF (P300/CBP-associated factor), CBP (cap binding protein), and p300. The isoform p47ING1a is found to inhibit histone acetylation and bind to histone deacetylase HDAC1 (histone deacetylase 1). The isoform p33ING1b regulates the association between proliferating cell nuclear antigen (PCNA) and p30 that is important for DNA repair and chromatin remodeling.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)