I1284

Isopropyl β-D-thiogalactopyranoside solution

ReadyMade IPTG solution for Blue-white screening

• Synonim(y): IPTG solution
• Wzór empiryczny (zapis Hilla): C₉H₁₈O₅S
• Numer CAS: 367-93-1
• Masa cząsteczkowa: 238.30
• Beilstein: 4631
• Numer MDL: MFCD00063273
• Kod UNSPSC: 12352200
• Identyfikator substancji w PubChem: 24895928
• NACRES: NA.52

Właściwości

• klasa czystości: Molecular Biology; for molecular biology
• Poziom jakości: 200
• opis: non-ionic
• Formularz: liquid
• masa cząsteczkowa: 238.3
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• ciąg SMILES: CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O
• InChI: 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
• Klucz InChI: BPHPUYQFMNQIOC-NXRLNHOXSA-N

Opis

Opis ogólny

Isopropyl thiogalactose (IPTG) is a sugar derivative widely used for the induction of the expression of recombinant proteins in E. coli. This expression system requires that the gene of interest is cloned in plasmids containing the Lac promoter. IPTG Ready Made is a 0.2 μm filter sterilized solution of 200 mM IPTG. This reagent remains liquid at its storage temperature (-20 °C), thus avoiding freeze-thaw cycles and providing a user-friendly solution.

Zastosowanie

Isopropyl β-D-thiogalactopyranoside solution is:

• used as an inducer for the expression of single domain antibodies(sdAbs) in E coli[1] and bioactive peptides[2]
• used in its half-life and decay studies[3]
• suitable for induction of recombinant protein expression in systems controlled by the lac repressor

Działania biochem./fizjol.

Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β--galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product. Since Isopropyl β-D-thiogalactopyranoside cannot be metabolized by E. coli its concentration remains constant. Therefore, it is not a variable in the experiment.[4]

Zasada

Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β-galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)