The lyophilized powder may be reconstituted at 5 mg/mL in 0.02M sodium phoshate buffer or 0.45% sodium chloride containing 0.01% BSA. Solutions prepared at concentrations of at least 1 mg/ml may be stored in aliquots at -20°C for several months.
H2126
Hyaluronidase from sheep testes
Type II, lyophilized powder, ≥300 units/mg solid
Synonim(y):
Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
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Wybierz wielkość
100 MG
323,00 zł
500 MG
963,00 zł
1 G
1650,00 zł
5 G
7820,00 zł
323,00 zł
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Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-464-3
MDL number:
Specific activity:
≥300 units/mg solid
Przejdź do
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Pozwól nam pomócconjugate
conjugate (Glucosaminoglycan)
type
Type II
form
lyophilized powder
specific activity
≥300 units/mg solid
mol wt
55 kDa
storage temp.
−20°C
Quality Level
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Powiązane kategorie
1 of 3
Ta pozycja | ||
|---|---|---|
| specific activity ≥300 units/mg solid | specific activity ≥300 units/mg dry wt. | specific activity ≥1,500 units/mg solid |
| form lyophilized powder | form powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 55 kDa | mol wt - | mol wt 55 kDa |
| Quality Level 200 | Quality Level 100 | Quality Level 200 |
| conjugate conjugate (Glucosaminoglycan) | conjugate conjugate (Glucosaminoglycan) | conjugate - |
General description
Hyaluronidase is a hyaluronic acid-metabolizing enzyme.[1] It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.[2]
Application
Hyaluronidase from sheep testes has been used:
- in flow cytometry[3]
- in the isolation of putative spermatogonial stem cells (SSCs)[4]
- to study the effects of magnetic stimulation in magnetic hydrogel properties[5]
- in immunohistochemistry to visualize collagen type II distribution[6][7]
- to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings[8]
- for the degeneration of hydrogels[9]
Biochem/physiol Actions
These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
Physical form
Lyophilized powder containing lactose
Other Notes
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Multifunctional magnetic-responsive hydrogels to engineer tendon-to-bone interface
Silva ED, et al.
Nanomedicine: Nanotechnology, Biology, and Medicine, 14(7), 2375-2385 (2018)
Side Effects of Drugs Annual: A world-wide yearly survey of new data and trends in adverse drug reactions., 603-619 (2010)
Suppression of Sertoli cell tumour development during the first wave of spermatogenesis in inhibin $\alpha$-deficient mice
Haverfield JT, et al.
Reproduction, Fertility, and Development, 29(3), 609-620 (2017)
Yield stress determines bioprintability of hydrogels based on gelatin-methacryloyl and gellan gum for cartilage bioprinting
Mouser VHM, et al.
Biofabrication, 8(3), 035003-035003 (2016)
Robert B Struijk et al.
PloS one, 15(3), e0230253-e0230253 (2020-03-17)
Autologous transplantation of spermatogonial stem cells is a promising new avenue to restore fertility in infertile recipients. Expansion of the initial spermatogonial stem cell pool through cell culturing is a necessary step to obtain enough cells for effective repopulation of
Produkty
Dowiedz się więcej o glikozoaminoglikanach i proteoglikanach, w tym o strukturze glikozoaminoglikanów (GAG), różnych typach GAG i ich funkcjach.
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Protokoły
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
Powiązane treści
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Kann die Hyaluronidase H2126 in PBS gelöst werden um sie dann in kleinen Aliquoten bei -20 C zu lagern?
1 answer-
Helpful?
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H2126-100MG Hyaluronidase from sheep testes についてですが、溶解方法を教えてください。
1 answer-
This enzyme is soluble at 1 mg/mL in 20 mM sodium phosphate buffer (pH 7.0), with 77 mM sodium chloride and 0.1 mg/mL BSA at 1 mg/mL. For assay purposes, this product is prepared at 0.3 mg/mL solution in Phosphate Buffer. Heating may be required, Please see the link below to review the full assay protocol:
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-hyaluronidase#preparationPlease see the link below to review the product datasheet for a bovine Hyaluronidase, product H3884. This information may also be applied to the ovine source material:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/739/183/h3884pis-ms.pdfHelpful?
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