H0413

HIS-Select^® Filter Plate

• Synonim(y): His-tag filtered microplate
• NACRES: NB.24
• UNSPSC Code: 41121800

Właściwości

• grade: Molecular Biology
• form: plate
• suitability: suitable for (cell culture)
• storage temp.: 2-8°C

Opis

General description

The HIS-Select^® Filter Plate is an immobilized metal-ion affinity chromatography (IMAC) product that allows rapid purification of small-scale crude cell extracts containing histidine-tagged proteins. The HIS-Select^® Filter Plate contains 20 μm, spherical silica particles (100 nm pore size) with a hydrophilic layer.

The HIS-Select^® Filter Plate is selective for recombinant proteins with histidine tags and exhibits very low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography. The binding capacity of the HIS-Select^® Filter Plate is typically > 2 mg per well as determined with an approximately 30 kDa histidine-tagged protein.

Application

The HIS-Select^® Filter Plate is suitable for purification of recombinant proteins with histidine tags.

Features and Benefits

• High Selectivity for his-tagged proteins
• Ready-to-use plate format
• Compatible with CelLytic^™ cell lysis reagents

Legal Information

Covered by US Patent No 6,623,655 and are sold for research use only. Commercial use requires additional licenses.

CelLytic is a trademark of Sigma-Aldrich Co. LLC

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H317,H334,H341,H350i,H360D,H372,H373,H412
• Ostrzeżenia: P202 - P260 - P273 - P280 - P302 + P352 - P308 + P313
• Hazard Classifications: Aquatic Chronic 3 - Carc. 1A Inhalation - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 - STOT RE 2 Inhalation
• Narządy docelowe: Respiratory Tract
• Klasa składowania: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable