This is a 10X stock solution of Grace′s amino acids modified for use in TC-100. Grace′s medium was originally formulated to support the growth of cells derived from the Australian emperor gum moth, Antherea eucalypti. The medium is a modification of Wyatt′s medium to more closely resemble Antherea hemolymph. The unparalled success achieved by Grace in maintaining long term cultures of a variety of insect cells led to a resurgence in the field of insect tissue culture and wide usage of the medium. Many modifications of this medium have been developed since its introduction.
Preparation Note
Z L-seryną. Bez β-alaniny, L-glutaminy i D-seryny.
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