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FG0325

Minimum Essential Medium Eagle

Name: Minimum Essential Medium Eagle
Brand: SIGMA

With Earle′s salts, stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonim(y):

EMEM, MEM

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Informacje o tej pozycji

UNSPSC Code:

12352207

NACRES:

NA.71

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Właściwości

Nazwa produktu

Minimum Essential Medium Eagle, With Earle′s salts, L-alanyl-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered, sterile

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

components

stable glutamine: yes
L-glutamine: no
phenol red: yes
sodium pyruvate: no
Earle’s salts (5% CO₂): yes
HEPES: no
Hanks’ salts (2% CO₂): no
glucose: yes
NaHCO₃: yes

shipped in

ambient

storage temp.

2-8°C

Opis

General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Application

Minimum Essential Medium Eagle has been used in murine fibroblast culture for in vitro cytocompatibility testing.

Other Notes

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln. It is supplemented with L-Ala-L-Gln dipeptide. This provides a more stable form of glutamine for cell culture. Free amino acid L-glutamine is known to be unstable in cell culture.

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Ta pozycja	M4655	M2414	M0325
SAFC FG0325 Minimum Essential Medium Eagle	Sigma-Aldrich M4655 Minimum Essential Medium Eagle	Sigma-Aldrich M2414 Minimum Essential Medium Eagle	Sigma-Aldrich M0325 Minimum Essential Medium Eagle
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
components stable glutamine: yes L-glutamine: no phenol red: yes sodium pyruvate: no Earle’s salts (5% CO₂): yes HEPES: no Hanks’ salts (2% CO₂): no glucose: yes NaHCO₃: yes	components sodium pyruvate: no phenol red: yes Earle’s salts (5% CO₂): yes HEPES: no NaHCO₃: yes L-glutamine: yes	components phenol red: 0.011 g/L NaHCO₃: 0.85 g/L glucose: 1.0 g/L L-glutamine: no	components glucose: 1.0 g/L (Dextro) L-glutamine: 0.292 g/L phenol red: 0.011 g/L Earle’s salts (5% CO₂): yes NaHCO₃: 2.2 g/L
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
impurities Endotoxin, tested	impurities endotoxin, tested	impurities endotoxin, tested	impurities Endotoxin, tested

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Klasa składowania

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certyfikaty analizy (CoA)

Lot/Batch Number

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