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Key Documents

F0926

Sigma-Aldrich

Fetal Bovine Serum

USDA approved, sterile-filtered, suitable for cell culture

Synonim(y):

Fetal Bovine Sera, Fetal Calf Sera, FBS, FCS, sera, serum

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About This Item

Numer MDL:
MFCD00132239
Kod UNSPSC:
12352207
NACRES:
NA.71

pochodzenie biologiczne

bovine fetus

Poziom jakości

500

sterylność

sterile-filtered

linia produktu

FBS Classic

jakość

USDA approved

skład

Hemoglobin, ≤25 mg/dL

metody

cell culture | mammalian: suitable

zanieczyszczenia

≤10 EU/mL endotoxin

Warunki transportu

dry ice

temp. przechowywania

−20°C

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Opis ogólny

Fetal bovine serum (FBS) or fetal calf serum is the blood fraction remaining after the natural coagulation of blood. It comes from the blood drawn from a bovine fetus via a closed system of collection at the slaughterhouse. It is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells.

Zastosowanie

FBS sourced from USDA approved non-USA countries is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factors to cells in culture.

Fetal Bovine Serum has been used for the following cell cultures:
  • Primary Culture Human Macrophages (mononuclear cells were re-suspended in Roswell Park Memorial Institute (RPMI) medium containing fetal bovine serum (FBS) along with other components).
  • Tissue Culture (MEFs were cultured in DMEM supplemented with FBS along with components).
  • Isolation and 3D culture of primary mammary epithelial organoids
  • Cell culture and medium (HUVECs were grown in Endothelial Cell Growth Medium-2 supplemented with FBS and growth factors)

Przydatność

USDA Approved Sourcing refers to FBS produced from blood collected in countries outside of the United States that have been approved by the USDA (United States Department of Agriculture) for export of beef products into the United States. Eligible countries for export into the U.S. include Australia, Canada, Chile, Costa Rica, Honduras, Iceland, Japan, Mexico, New Zealand, Nicaragua, Uruguay. Foreign serum is tested to ensure the absence of exotic viruses before release by the USDA.

Komentarz do analizy

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Inne uwagi

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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Sigma-Aldrich

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Chroń swoją inwestycję dzięki najlepszym praktykom przechowywania i rozmrażania FBS i innych surowic. Próbki FBS do walidacji w protokole hodowli komórkowej.

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