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DUO92004

Sigma-Aldrich

Duolink® In Situ PLA® Probe Anti-Mouse MINUS

Affinity purified Donkey anti-Mouse IgG (H+L)

Synonim(y):

in situ Proximity Ligation Assay reagent, Protein Protein Interaction Assay reagent

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.32

pochodzenie biologiczne

donkey (polyclonal)

Poziom jakości

200

forma przeciwciała

affinity purified immunoglobulin (secondary antibody)

rodzaj przeciwciała

primary antibodies

linia produktu

Duolink®

reaktywność gatunkowa

mouse

metody

immunofluorescence: suitable
proximity ligation assay: suitable

przydatność

suitable for brightfield
suitable for fluorescence

Warunki transportu

wet ice

temp. przechowywania

2-8°C

Powiązane kategorie

Opis ogólny

Duolink® In Situ PLA® Probe Anti-Mouse MINUS contains affinity purified donkey anti-mouse IgG (H+L), which reacts with whole molecule mouse IgG and also reacts with the light chains of other mouse immunoglobulins.

Zastosowanie

Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

This product can be applied to both the Duolink® In Situ Fluorescence Protocol and the Duolink® In Situ Brightfield Protocol depending on the detection reagents used.

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.
Specificity
PLA probe anti-Mouse reacts with whole molecule Mouse IgG and the light chains of other Mouse immunoglobulin?s. The PLA probe anti-Mouse may cross-react with Rat antibodies, but has minimal cross reactivity with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, and sheep serum proteins. A PLUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink®PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

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Cechy i korzyści

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Komponenty

This product is comprised of the following:
  • 5x PLA Probe Anti-Mouse MINUS - Donkey anti-mouse secondary antibody conjugated to oligonucleotide MINUS
  • 1x Blocking Solution - Reagent for blocking of the sample
  • 1x Antibody Diluent - For dilution of PLA probes and primary antibodies
See datasheet for more information.

Uwaga dotycząca przygotowania

Storage and Stability: Store the PLA Probe Anti-Mouse MINUS at 2–8°C. Do not store the diluted PLA Probe Anti-Mouse MINUS solution.

Inne uwagi

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Informacje prawne

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

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Piktogramy

Exclamation markEnvironment
GHS07,GHS09

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H317,H411

Zwroty wskazujące środki ostrożności

P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313

Klasyfikacja zagrożeń

Aquatic Chronic 2 - Skin Sens. 1

Kod klasy składowania

12 - Non Combustible Liquids

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Novel function of ceramide for regulation of mitochondrial ATP release in astrocytes.
Kong J N, et al.
Journal of Lipid Research, M081877-M081877 (2018)
Proximal Ligation Assay (PLA) on Lung Tissue and Cultured Macrophages to Demonstrate Protein-protein Interaction.
Mendez R and Santanu B
Bio-protocol, 7(21) (2017)
Interaction of the Mouse Polyomavirus Capsid Proteins with Importins Is Required for Efficient Import of Viral DNA into the Cell Nucleus.
Soldatova I, et al.
Viruses, 10(4), 165-165 (2018)
Roberto Di Maio et al.
Science translational medicine, 10(451) (2018-07-27)
Missense mutations in leucine-rich repeat kinase 2 (LRRK2) cause familial Parkinson's disease (PD). However, a potential role of wild-type LRRK2 in idiopathic PD (iPD) remains unclear. Here, we developed proximity ligation assays to assess Ser1292 phosphorylation of LRRK2 and, separately
M Aubele et al.
British journal of cancer, 103(5), 663-667 (2010-08-12)
Protein tyrosine kinase 6 (PTK6; breast tumour kinase) is overexpressed in up to 86% of the invasive breast cancers, and its association with the oncoprotein human epidermal growth factor receptor 2 (HER2) was shown in vitro by co-precipitation. Furthermore, expression
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Protein-protein Interactions Detection with PLA

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Protokoły

Duolink® PLA Brightfield Protocol

Duolink® PLA reagents enable brightfield detection and quantification of proteins and interactions in tissue samples.

Powiązane treści

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Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay

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