DUO92004

Duolink^® In Situ PLA^® Probe Anti-Mouse MINUS

Affinity purified Donkey anti-Mouse IgG (H+L)

• NACRES: NA.32
• UNSPSC Code: 12352203
• Species reactivity: mouse
• Technique(s): immunofluorescence: suitable, proximity ligation assay: suitable
• Application: IF, PLA
• Citations: 672

Właściwości

• biological source: donkey (polyclonal)
• Quality Segment: 200
• antibody form: affinity purified immunoglobulin (secondary antibody)
• antibody product type: primary antibodies
• product line: Duolink^®
• species reactivity: mouse
• technique(s): immunofluorescence: suitable, proximity ligation assay: suitable
• suitability: suitable for brightfield, suitable for fluorescence
• shipped in: wet ice
• storage temp.: 2-8°C

Opis

General description

Duolink^® In Situ PLA^® Probe Anti-Mouse MINUS contains affinity purified donkey anti-mouse IgG (H+L), which reacts with whole molecule mouse IgG and also reacts with the light chains of other mouse immunoglobulins.

Application

Specificity
PLA probe anti-Mouse reacts with whole molecule Mouse IgG and the light chains of other Mouse immunoglobulin?s. The PLA probe anti-Mouse may cross-react with Rat antibodies, but has minimal cross reactivity with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, and sheep serum proteins. A PLUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^®PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

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Duolink^®proximity ligation assay(PLA^®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

This product can be applied to both the Duolink^® In Situ Fluorescence Protocol and the Duolink^® In Situ Brightfield Protocol depending on the detection reagents used.

To perform a complete Duolink^® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink^® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.

Features and Benefits

• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

Preparation Note

Storage and Stability: Store the PLA Probe Anti-Mouse MINUS at 2–8°C. Do not store the diluted PLA Probe Anti-Mouse MINUS solution.

Other Notes

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This product is comprised of the following:

• 5x PLA Probe Anti-Mouse MINUS - Donkey anti-mouse secondary antibody conjugated to oligonucleotide MINUS
• 1x Blocking Solution - Reagent for blocking of the sample
• 1x Antibody Diluent - For dilution of PLA probes and primary antibodies

See datasheet for more information.

Legal Information

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany

PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• pictograms: GHS07,GHS09
• signalword: Warning
• hcodes: H317,H411
• pcodes: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Hazard Classifications: Aquatic Chronic 2 - Skin Sens. 1
• Klasa składowania: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• wgk: WGK 3