Przejdź do zawartości
Merck
Wszystkie zdjęcia(1)

Dokumenty

DUO87009

Sigma-Aldrich

Duolink® PLA® Probe Mouse Anti-PhosTyr PLUS from mouse

Protein Phosphorylation Detection by Proximity Ligation Assay

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wszystkie zdjęcia(1)

About This Item

Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

200

forma przeciwciała

purified antibody (Protein G-Sepharose)

rodzaj przeciwciała

primary antibodies

klon

4G10®, monoclonal

linia produktu

Duolink®

reaktywność gatunkowa

all

metody

proximity ligation assay: suitable

przydatność

suitable for brightfield
suitable for fluorescence

Warunki transportu

wet ice

temp. przechowywania

2-8°C

Opis ogólny

Anti-Phosphotyrosine antibody, clone 4G10® with the Duolink PLA PLUS oligonucleotide. This antibody detects tyrosine phosphorylated proteins in all species. Protein target specificity can be obtained by pairing with an antibody specific to a protein of interest in a Duolink PLA experiment. This product must be used in combination with a MINUS PLA probe of a different species or another directly-conjugated primary antibody with a MINUS PLA oligo. The Anti-mouse PLA probe PLUS (DUO92001) is not necessary when using this product.

Specyficzność

This directly-conjugated primary antibody PLA probe recognizes tyrosine-phosphorylated proteins from all species.

Zastosowanie

Duolink® proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

This product can be applied to both the Duolink® In Situ Fluorescence Protocol and the Duolink® In Situ Brightfield Protocol depending on the detection reagents used.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.

Application Note
Protein phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. Anti-phosTyr antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosine phosphorylation of the protein of interest. This process is time-consuming and cannot provide subcellular localization.

Anti-phosTyr PLA probes are used to detect the phosphorylation of tyrosine residues in proteins. Protein target specificity can be obtained by pairing with an antibody specific to a protein of interest in a Duolink® PLA® experiment. This product is particularly useful when site-specific anti-phosphorylation antibodies are not available. A MINUS PLA probe of a different species or another directly-conjugated primary antibody with a MINUS PLA oligo must be used in combination with this product. The Anti-mouse PLA probe PLUS (DUO92001) is not necessary when using this product. See our Product Selection Guide for more information.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

Cechy i korzyści

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Informacje prawne

4G10 is a registered trademark of Upstate Group, Inc.
Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
This page may contain text that has been machine translated.

Nie możesz znaleźć właściwego produktu?  

Wypróbuj nasz Narzędzie selektora produktów.

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej