This kit provides sufficient reagents to perform 18 chloroplast isolation procedures, using 30 grams of starting plant tissue, as described in the protocol. Please see the link below to review the product datasheet:_x000D_
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/114/229/cpisopis-ms.pdf
CPISO
Chloroplast Isolation Kit
isolation of intact chloroplasts from leaves
Synonim(y):
Chloroplast Purification Kit, Isolation Kit for Chloroplast, Kit for Chloroplast Isolation
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Wybierz wielkość
1 KIT
3800,00 zł
3800,00 zł
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Informacje o tej pozycji
NACRES:
NA.32
UNSPSC Code:
12352200
Przejdź do
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Pozwól nam pomóc1 of 4
Ta pozycja | |||
|---|---|---|---|
| technique(s) centrifugation: suitable | technique(s) ChIP: suitable | technique(s) DNA purification: suitable | technique(s) DNA purification: suitable |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 15-25°C | storage temp. 15-25°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
The Chloroplast Isolation Kit provides an efficient procedure for isolating intact chloroplasts from plant leaves. The procedure involves mechanical cell wall and cell membrane breakage, removal of cell debris and leaf tissue by filtration, collection of total cell chloroplast fraction by centrifugation, and separation of intact chloroplasts from broken chloroplasts using a Percoll® layer or gradient.
Application
Chloroplast Isolation Kit may be used in chloroplast organelle research to isolate intact chloroplasts from plant leaves for structural and functional studies.
The Chloroplast Isolation Kit has been tested for use with spinach, pea, lettuce, cabbage, mangold, and tobacco.
Features and Benefits
- Specifically formulated buffer - Saves time using easy to follow simple and proven protocols
- Isolates intact chloroplasts - Useful for studies of such processes as carbon assimilation, electron flow and phosphorylation, metabolic transport or protein targeting
- Total organelle protein complement - Useful for functional studies, metabolic transport, proteomic profiling, and genomic applications
- Convenient format - Saves time and reduces waste
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Klasa składowania
10 - Combustible liquids
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
The Complete Chloroplast Genome of 17 Individuals of Pest Species <I>Jacobaea vulgaris</I>: SNPs, Microsatellites and Barcoding Markers for Population and Phylogenetic Studies.
Doorduin, L., et al.
DNA Res., 1-13 (2011)
Xiaoli Luo et al.
PloS one, 8(1), e54002-e54002 (2013-01-22)
In plants, CuZn superoxide dismutase (CuZnSOD, EC l.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11), and catalase (CAT, EC l.11.1.6) are important scavengers of reactive oxygen species (ROS) to protect the cell from damage. In the present study, we isolated three homologous
Esau Megias et al.
The protein journal, 37(3), 290-299 (2018-05-29)
Tymovirus is a genus of plant pathogenic viruses that infects several dicotyledonous plants worldwide, causing serious diseases in economically important crops. The known cytopathic effect on the host cell organelles involves chloroplast membrane deformation and the induction of vesicles in
Jimena Cascales et al.
Life (Basel, Switzerland), 7(4) (2017-11-23)
The use of molecular markers with inadequate variation levels has resulted in poorly resolved phylogenetic relationships within Ilex. Focusing on southern South American and Asian species, we aimed at contributing informative plastid markers. Also, we intended to gain insights into
Martha Sainz et al.
Physiologia plantarum, 140(1), 46-56 (2010-05-22)
Drought and heat stress have been studied extensively in plants, but most reports involve analysis of response to only one of these stresses. Studies in which both stresses were studied in combination have less commonly been reported. We report the
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| CPISO-1KT | 04061833532775 |
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