CLLS1003

DLD1 Cells PIK3CA +/-

human male colon (Source Disease: Colorectal adenocarcinoma)

• Kod UNSPSC: 41106514
• NACRES: NA.81

Właściwości

• Nazwa produktu: DLD1 Cells PIK3CA +/-,
• pochodzenie biologiczne: human male colon (Source Disease: Colorectal adenocarcinoma)
• numer dostępu OMIM: 171834
• temp. przechowywania: −196°C
• informacje o genach: human ... PIK3CA(5290)

Opis

Opis ogólny

DLD1 Cells PIK3CA +/- are colorectal adenocarcinoma, epithelial cells, from a human adult male, with a ZFN knock out modification.

Zastosowanie

Media Renewal changes two to three times per week

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3 to 1:10

The base medium for this cell line is RPMI, Cat. No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%.

The cell freezing medium is DMSO 1X (Cat. No. C6164)

Cechy i korzyści

These DLD-1 cells are adherent, with a doubling time of approx. 20 hours.

Zinc finger nuclease (ZFN) knock out on chromosome 3q23.3

Jakość

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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Elementy zestawu są też dostępne oddzielnie

• DLD1 Control Cells 1 vial
• DLD1 Cells PIK3CA +/- 1 vial

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable