MFE-296

98031101, human endometrium, Epithelial

• Synonim(y): MFE296 Cells
• Kod UNSPSC: 41106514

Właściwości

• product name: MFE-296, 98031101
• pochodzenie biologiczne: human endometrium
• tryb wzrostu: Adherent
• kariotyp: Near Tetraploid
• morfologia: Epithelial
• produkty: Not specified
• receptory: Androgen receptor
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human caucasian endometrial adenocarcinoma

Opis linii komórkowej

MFE-296 was obtained from a moderately differentiated human endometrial adenocarcinoma. The cells were shown to express functionally intact androgen receptors. Proliferation was inhibted by progestin R5020 and dihydrotestosterone (DHT), the latter one being antagonised by the antiandrogen Casodex. MFE-296 express vimentin and the cytokeratins 7, 8, 18 and 19. Karyotype analysis revealed near tetraploidy for most cells. Further more, the cells have been shown to be tumorigenic in nude mice.

Zastosowanie

Tumour biology studies, receptor analysis

Profil DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8,13
D16S539: 11,14
D5S818: 11,13
D7S820: 8,10
THO1: 6,9.3
TPOX: 8
vWA: 14,18

pożywka hodowlana

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding 1-2 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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