DCH-008 human cell line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: human blood
• Technique(s): cell culture | mammalian: suitable
• Ethnicity: Thai
• Gender: male
• Growth mode: Suspension
• Morphology: Lymphoblastoid

Właściwości

• biological source: human blood
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 94082284-DNA-5UG, pkg of vial of cells 94082284-1VL
• ethnicity: Thai
• gender: male
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblastoid
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: -140 to -196°C

Opis

General description

Consanguineous: No

Homozygous: No

Biochem/physiol Actions

A: 11 24
B: 1512/19 1502
HLA DRB1: 09 1202
HLA DRB3: 301
HLA DRB4: 101
HLA DQAP: 3.2 4.2
HLA DQA1: 0302 0601
HLA DQB1: 0301 0303
HLA DPB1: 0301 4101

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Preparation Note

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

HLA A: 11 24
HLA A ief: 11.2 24.1
HLA C: 9 8
HLA B: 75 76

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Disclaimer

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

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