BYD, A human cell line

940822106, IHW Number: 9202

• Kod UNSPSC: 41106514

Właściwości

• pochodzenie biologiczne: human blood
• pochodzenie etniczne: Caucasian
• płeć: female
• tryb wzrostu: Suspension
• kariotyp: Not specified
• morfologia: Lymphoblastoid
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice

Opis

Opis ogólny

Consanguineous: No

Homozygous: No

Opis linii komórkowej

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Profil DNA

A: 03 02
B: 35 50
HLA DRB1: 0301 1104
HLA DRB3: 0101 0202
HLA DQAP: 4.1
HLA DQA1: 501
HLA DQB1: 0201 0301
HLA DPB1: 02012 0301

Profil serologiczny

HLA A: 3 2
HLA A ief: 3.2 2.2
HLA B: 35 21
HLA B ief:

pożywka hodowlana

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Rutyna subkultury

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Inne uwagi

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