IDF human cell line

94022530, IHW Number: 9211

• Kod UNSPSC: 41106514

Właściwości

• pochodzenie biologiczne: human blood
• pochodzenie etniczne: Ashkenasi Jewish
• płeć: male
• tryb wzrostu: Suspension
• kariotyp: Not specified
• morfologia: Lymphoblastoid
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice

Opis

Opis ogólny

Consanguineous: No

Homozygous: No

Opis linii komórkowej

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Profil DNA

A: 6901 6601 (!)68
Cw: 1203
B: 18 3801
HLA DRB1: 11
HLA DRB3: 202
HLA DQA1: 501
HLA DQB1: 301
HLA DPB1: 0201 0401

Profil serologiczny

HLA A: 26 69
HLA B: 38 18
Bw4/bw6: 4 6
HLA Dw: 5
HLA DR: 11
HLA DR52: 52
HLA DQ: 7
HLA DP: 4

pożywka hodowlana

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Rutyna subkultury

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Inne uwagi

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Oświadczenie o zrzeczeniu się odpowiedzialności

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.